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| Isolation of a Cd-resistant bacterium and its effect on the speciation of Cd in soil |
| Received:July 10, 2017 Revised:November 06, 2017 |
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| KeyWord:Delftia acidovorans;Cd;adsorption rate;speciation |
| Author Name | Affiliation | E-mail | | LIU Yu-ling | College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China
Engineering Research Center of Efficient Utilization of Heavy Metal Polluted Crop-land, Hunan Province, Changsha 410013, China | | | TIE Bai-qing | College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China
Engineering Research Center of Efficient Utilization of Heavy Metal Polluted Crop-land, Hunan Province, Changsha 410013, China | tiebq@qq.com | | LI Yuan-xing-lu | College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China
Engineering Research Center of Efficient Utilization of Heavy Metal Polluted Crop-land, Hunan Province, Changsha 410013, China | | | WEI Xiang-dong | College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China
Engineering Research Center of Efficient Utilization of Heavy Metal Polluted Crop-land, Hunan Province, Changsha 410013, China | | | PENG Ou | College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China
Engineering Research Center of Efficient Utilization of Heavy Metal Polluted Crop-land, Hunan Province, Changsha 410013, China | | | YE Chang-cheng | College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China
Engineering Research Center of Efficient Utilization of Heavy Metal Polluted Crop-land, Hunan Province, Changsha 410013, China | | | LIU Xiao-li | College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China
Engineering Research Center of Efficient Utilization of Heavy Metal Polluted Crop-land, Hunan Province, Changsha 410013, China | | | SUN Jian | School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China | |
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| Abstract: |
| Contamination of heavy metals has been increasing in recent years due to industrial activity. Consequently, microbial remediation of heavy metal-polluted soils has received increasing interest. A bacterial strain, B9, with high cadmium adsorption capacity was isolated from cadmium-polluted soils through a conventional method of bacterial isolation and cultivation, and the effects of the strain on soil Cd speciation were investigated by a pot experiment to evaluate the feasibility of using B9 in Cd bioremediation. The results of 16S rRNA gene sequence analysis showed that B9 was 99% homologous to Delftia acidovorans, and was identified as Delftia sp. by morphological and biochemical analysis. It was found that the highest adsorption efficiency for Cd2+ was achieved at pH 8, 35℃, and 48 h of incubation time. In these conditions, more than 60% of the Cd2+ could be adsorbed from a concentration below 10 mg·L-1. B9 could facilitate Cd transformation from weak acid-soluble to reducible and residual phases in soil. The addition of 3 mL and 10 mL bacterial suspensions into the soil resulted in 22.17% and 25.06% decreases in the content of weak acid-soluble Cd, 9.66% and 12.17% increases in the content of reduced Cd, and 13.55% and 13.61% increases in the content of residual Cd, respectively, whereas the content of oxidized Cd did not significantly change. The results provide convincing evidence for the potential application of the B9 strain for bioremediation of Cd-contaminated soil. |
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