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| Influence of lead on bacterial community composition in Lou soil |
| Received:July 08, 2017 Revised:November 15, 2017 |
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| KeyWord:16S rDNA gene sequence;bacteria;Lou soil;lead;bacterial community |
| Author Name | Affiliation | E-mail | | AN Feng-qiu | College of Natural Resources and Environment, Northwest A & F University, Ministry of Agriculture Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Yangling 712100, China
College of Environmental and Chemical Engineering, Xi'an Polytechnic University, Xi'an 710048, China | | | LÜ Jia-long | College of Natural Resources and Environment, Northwest A & F University, Ministry of Agriculture Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Yangling 712100, China | ljlll@nwafu.edu.cn | | DIAO Zhan | College of Natural Resources and Environment, Northwest A & F University, Ministry of Agriculture Key Laboratory of Plant Nutrition and Agri-environment in Northwest China, Yangling 712100, China | | | LI Hai-hong | College of Environmental and Chemical Engineering, Xi'an Polytechnic University, Xi'an 710048, China | | | ZHAO Qi-qi | College of Environmental and Chemical Engineering, Xi'an Polytechnic University, Xi'an 710048, China | |
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| Abstract: |
| To research the soil bacteria microbial community composition influenced by lead, while examining its relationship with the physicochemical properties of Pb-contaminated Lou soils, the Illumina platform Miseq high-throughput sequencing technique was used to analyze the agricultural soil treated with various concentrations of Pb2+. Three treatments, namely high concentration treatment(WHIGH), low concentration treatment(WLOW), and control(WCK), were used. The results showed that Proteobacteria(with an average relative abundance of 25%) and Actinobacteria(with an average relative abundance of 18.6%) were the most abundant phyla among the three treatments. The other dominant phyla were Acidobacteria, Bacteroidetes, etc. Actinobacteria, Betaproteobacteria, and Cytophagi were the most abundant classes in WHIGH among the three treatments. At the genus level, the relative abundance of 9 genera were significantly different among the three treatments(P<0.05), namely Pseudomonas, Skemanella, Balneimona, Agromyces, Enhydrobacter, Devosia, Variovorax, Flavihumibacter and Lacibacter. Moreover, the relative abundance of 7 genera were below 0.3%, except for Pseudomonas and Skemanella in both treatments. The variance analysis showed that the richness index of the community(Chao and ACE) had significant differences between the WHIGH and WLOW treatments(P<0.05). The bacteria community structure of OTU was fairly similar in the WCK and WLOW treatments, while it was different in the WHIGH treatment. The redundancy analysis and correlation analysis showed that the content of available Pb and total Pb were positively correlated with WHIGH's bacterial community structure of OTU, while being negatively correlated with those of WLOW and WCK. Firmicutes, Gemmatimonadetes, Verrucomicrobia,Chloroflexi, and Nitrospirae correlated closely with the physicochemical properties of soil(P<0.05). The results of the present study indicated that bacterial community of Lou soil has been greatly influenced by long-term Pb contamination, which changed the bacterial community composition of the soil. |
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