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| Immobilized sulfate reducing bacteria beads using microalgae as nutrient source for effective biotreatment of copper-containing wastewater in an upflow anaerobic reactor |
| Received:August 08, 2017 Revised:October 16, 2017 |
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| KeyWord:sulfate reducing bacteria;immobilization;microalgae;copper-containing wastewater |
| Author Name | Affiliation | E-mail | | LI Yong-chao | School of Civil Engineering, Hunan University of Science and Technology, Xiangtan 411201, China | | | YANG Xiao-yan | Agricultural Clear Watershed Group, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China | | | LI Yan-ling | Agricultural Clear Watershed Group, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China | | | GENG Bing | Agricultural Clear Watershed Group, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China | gengbing2000@126.com |
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| Abstract: |
| Genus Desulfovibrio sp., belonging to the sulfate-reducing bacteria(SRB) strain, was isolated from a sludge sample. Degradable microalgae(Chlorella vulgaris, Scenedesmus obliquus, Selenastrum capricornutum, and Anabaena spiroides) were first selected as carbon sources for SRB. Then, immobilized SRB beads were prepared using polyvinyl alcohol and sodium alginate as a conventional gel matrix, and the embedding condition was optimized through an orthogonal experiment. These beads were used for the treatment of copper-containing wastewater in an upflow anaerobic reactor. Results showed that under the action of co-existing fermentative bacteria, the microalgae were first degraded to fatty acids, which could serve as a carbon source for SRB. In particular, Scenedesmus obliquus was adopted as the carbon source because its fermentation products were better. The optimal parameters for preparing immobilized beads with higher sulfate reduction rate are as follows:polyvinyl alcohol 2%, sodium alginate 1%, calcium chloride 6%, silica sand 1%, and 50 mL volume of SRB suspension. During the initial stage of the reaction, pollutant removal in the upflow anaerobic reactor was mainly due to the adsorption of immobilized SRB beads. Five days later, biological sulfate reduction played a dominant role, and Cu2+ removal rate reached more than 98% during 0~36 days of reaction. After 45 days, the reactor ran out. In particular, the Cu2+ removal ability for microalgae was 45.28 mg·d-1·g-1 and the SO42- removal ability was about 182.17 mg·d-1·g-1. |
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