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| Effects of EDDS on growth, antioxidant enzyme, and Cd accumulation of Bidens pilosa L. seedlings under Cd stress |
| Received:August 16, 2017 |
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| KeyWord:EDDS;Cd stress;Bidens pilosa L.;growth;antioxidant enzyme;Cd accumulation |
| Author Name | Affiliation | E-mail | | YANG Bo | School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China | | | CHEN Yin-ping | School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China | yinpch@mail.lzjtu.cn | | KE Yun-qi | School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China | | | YAN Zhi-qiang | School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China | | | YU Pei-dong | School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China | | | SHI Xiao-ping | School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China | |
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| Abstract: |
| A pot experiment was conducted to study the effects of adding 0(CK), 0.5, 1.5, 2.5 mmol·L-1, and 5.0 mmol·L-1 ethylenediamine disuccinic acid(EDDS) on growth, antioxidant enzyme activities, and Cd accumulation in Bidens pilosa seedlings under 40 mg·kg-1 Cd stress. The results showed that the dry and fresh weight, plant height, and root length of the seedlings were promoted significantly by treatments with 0.5 mmol·L-1 and 1.5 mmol·L-1 EDDS. The belowground(roots) and aboveground(stems and leaves) parts had significant higher level of Cd accumulation in the 0.5, 1.5 mmol·L-1, and 2.5 mmol·L-1 EDDS treatments than that in the CK, but the concentration of 1.5 mmol·L-1 showed the best effect, with the maximum value of 31.954 mg·kg-1 and 109.454 mg·kg-1 in the belowground and aboveground parts, respectively. The highest bioaccumulation factor(3.521) and the highest translocation factor(3.426) were also observed in the 1.5 mmol·L-1 EDDS treatment. With increasing EDDS concentration, activity of catalase(CAT), ascorbate peroxidase(APX), glutathione reductase(GR), peroxidase(POD) and superoxide dismutase(SOD) in the belowground and aboveground parts of Bidens pilosa seedlings under Cd stress appeared to present an initial increasing trend, followed by a decrease or persistent increase. These findings indicated that the antioxidant enzyme system was activated to eliminate reactive oxygen species(ROS) and alleviate membrane lipid peroxidation damage induced by Cd stress. The present results suggested that an appropriate concentration of EDDS could promote the growth of seedlings under Cd stress and increase the capacity for absorption and accumulation of Cd, which is beneficial for the remediation of Cd-contaminated soil. Taking into account the cost of chelating agent and secondary soil pollution caused by the chelating agent, the appropriate concentration of EDDS was found to be 1.5 mmol·L-1. |
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