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| The screening-out and degradation character of an indigenous benzo[a]pyrene-degrading bacteria |
| Received:July 06, 2017 |
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| KeyWord:polycyclic aromatic hydrocarbons (PAHs);benzo[a]pyrene (B[a]P);contaminated soil;biodegradation |
| Author Name | Affiliation | E-mail | | GU Ping | Heilongjiang Provincial Environmental Monitoring Center Station, Harbin 150056, China
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China | | | ZHOU Qi-xing | Key Laboratory of Pollution Processes and Environmental Criteria Ministry of Education/Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China | zhouqx@nankai.edu.cn | | WANG Xin | Key Laboratory of Pollution Processes and Environmental Criteria Ministry of Education/Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China | | | WU Yue-hui | Heilongjiang Provincial Environmental Monitoring Center Station, Harbin 150056, China | | | CHEN Wei | Heilongjiang Provincial Environmental Monitoring Center Station, Harbin 150056, China | | | ZHANG Qian-ru | Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China | | | GONG Qi | Environmental Emergency and Accident Investigation Center of Heilongjiang Province, Harbin 150090, China | | | ZHOU Ai-shen | Heilongjiang Provincial Environmental Monitoring Center Station, Harbin 150056, China | |
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| Abstract: |
| Eight bacteria strains were isolated from the soil contaminated with petroleum hydrocarbons and polycyclic aromatic hydrocarbons(PAHs) using repeated subcultures in liquid medium with added benzo[a]pyrene(B[a]P) as the sole carbon source. One of the bacterial strains named BB-1 had an optimal degradation effect(100% homologous to Bacillus megaterium) through the 16sRNA gene sequencing method. In order to explore the degradation character of BB-1,the strain was cultured under pH=7.0, 30℃ for 8 days, after which the B[a]P degradation rate reached a maximum of 66.36%. In order to investigate the degradation rate of BB-1 under varying conditions of initial pH and additional sucrose concentration in the culture medium, the incubation was carried out at 30℃ for 8 days, with various values of initial pH including 4.0, 6.0, 8.0, and 10.0. The results showed that the degradation rates of B[a]P were 12.14%, 39.61%, 55.21%, and 30.03%, respectively. In other words, the highest degradation efficiency was achieved for pH 8.0. With the addition of 0.1% and 0.5% sucrose as an additional carbon source, the degradation rate of B[a]P reached 70.56% and 74.89%, respectively. It was thus clear that the highest degradation efficiency occurred in the media with the addition of 0.5% sucrose. |
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