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Isolation, identification, and characterization of atrazine-degrading bacterial strain CS3
Received:October 25, 2017  
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KeyWord:atrazine;Arthrobacter sp.;biodegradation
Author NameAffiliationE-mail
YANG Xiao-yan Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Science, Beijing 100081, China  
LI Yan-ling Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Science, Beijing 100081, China  
WEI Huan-yu Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Science, Beijing 100081, China  
ZHU Chang-xiong Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Science, Beijing 100081, China  
LI Feng Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Science, Beijing 100081, China  
GENG Bing Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Science, Beijing 100081, China gengbing2000@126.com 
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Abstract:
      Isolation of microbial resources from polluted environments used for soil remediation will be increasingly necessary in future agriculture. In view of this, atrazine-degrading bacterial strain CS3, which grows using atrazine as its sole nitrogen source, was isolated from the effluent of a pesticide factory in Hebei Province. Based on its biochemical characterization and 16S rRNA gene sequence, strain CS3 was identified as Arthrobacter unreafaciences. Its optimum growth temperature and pH were 30℃ and 7.0, respectively. Under these conditions, A. unreafaciences CS3 completely degraded 50 mg·L-1 atrazine within 48 h and 500 mg·L-1 within 6 days. A. unreafaciences CS3 contains the atrazine-degrading genes trzN, atzB, and atzC and has good growth and atrazine degradation in the pH range 5~11.