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| Effects of three microorganisms on fluorescence physiology and accumulative characteristics of Eichhornia crassipes under uranium stress |
| Received:March 30, 2018 |
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| KeyWord:Eichhornia crassipes;microorganisms;uranium;fluorescent physiology;accumulative characteristics |
| Author Name | Affiliation | E-mail | | DENG Wen-yang | Life Science and Engineering College, Southwest University of Science and Technology, Mianyang 621010, China | | | LUO Xue-gang | State Defense Key Laboratory of the Nuclear Waste and Environmental Security, Southwest University of Science and Technology, Mianyang 621010, China | lxg@swust.edu.cn | | LUO Lan | Environment and Resources College, Southwest University of Science and Technology, Mianyang 621010, China | | | WANG Zhuo | Life Science and Engineering College, Southwest University of Science and Technology, Mianyang 621010, China | |
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| Abstract: |
| A greenhouse hydroponics experiment was conducted to explore the influence of microorganisms on the phytoremediation of U pollution and to obtain a better bioremediation model for uranium. The effect of three kinds of microorganism(Bacillus subtilis, Bacillus mucilaginosus, and Aspergillus niger)inoculated with different U concentrations(0, 5, 15, 25 mg·L-1)on the biomass, fluorescence physiology, and U enrichment characteristics in Eichhornia crassipes were studied. The results showed that all the three kinds of microorganisms could promote the growth of Eichhornia crassipes under U stress. When the U concentration was 25 mg·L-1, Bacillus subtilis increased the root dry weight of Eichhornia crassipes by 34.8% and Bacillus mucilaginosus increased the dry weight of the stems and leaves by 60.5%. However,Aspergillus niger inhibited the activity of the three antioxidant enzymes of Eichhornia crassipes, while Bacillus subtilis and Bacillus mucilaginosus promoted them. All three microorganisms increased the maximum photochemical efficiency(FV/Fm)of Eichhornia crassipes. However, the light and performance index(PIabs)and variable fluorescence intensity(Vj)only obviously increased at 5 mg·L-1 and 15 mg·L-1. Furthermore, under the conditions of the experiment, all three microorganisms had a significant effect on the ability of Eichhornia crassipes to enrich U. When the concentration of U was 25 mg·L-1, Bacillus mucilaginosus increased the enrichment concentration of U in the root of Eichhornia crassipes by 36.6%. Bacillus subtilis increased the U enrichment concentration in the stems and leaves of Eichhornia crassipes by 14.1%. In addition, all three microorganisms facilitated the enrichment amount and concentration of U in Eichhornia crassipes. The most obvious effect was Bacillus mucilaginosus to U enrichment amount, which showed a 51.8% increase. Bacillus subtilis distinctly improved the translocation factor(TF)of Eichhornia crassipes by up to 1.192, while Aspergillus niger increased the bioconcentration factor(BCF)significantly, to a maximum of 272.8. Inoculation with microorganisms in the roots of plants may affect the phytoremediation process. Inoculation with Bacillus subtilis, Bacillus mucilaginosus, or Aspergillus niger in the roots of Eichhornia crassipes can increase its biomass and U enrichment capacity. |
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