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| Effects of EDTA and resistant bacteria on the uptake of Pb and Cd by ryegrass grown in Pb and Cd-contaminated soil |
| Received:October 05, 2017 |
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| KeyWord:ryegrass;resistant bacteria;EDTA;heavy metals;joint repair |
| Author Name | Affiliation | E-mail | | SHI Ding-ding | Agricultural College, Guangxi University, Nanning 530004, China | | | LIANG Xiao-di | Agricultural College, Guangxi University, Nanning 530004, China | | | XU Shao-hui | Agricultural College, Guangxi University, Nanning 530004, China | | | JIANG Dai-hua | Agricultural College, Guangxi University, Nanning 530004, China | dhjiang2008@gxu.edu.cn | | HUANG Zhi-gang | Agricultural College, Guangxi University, Nanning 530004, China | |
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| Abstract: |
| A soil culture test and laboratory analysis was conducted to compare the remediation effect of EDTA and resistant bacteria(strain number J6)on the uptake of Pb and Cd by ryegrass in contaminated soil. A two-factor randomized block design was used, and the first factor was the concentration of Cd and Pb. We set six levels(Cd and Pb levels 0, 0 mg·kg-1; 5, 50 mg·kg-1; 10, 100 mg·kg-1; 20, 200 mg·kg-1; 50, 400 mg·kg-1; and 80, 800 mg·kg-1, respectively), coded A1, A2, A3, A4, A5, and A6. The second factor was different enhancers, which were EDTA, J6 bacteria, and blank. Our results showed that under the conditions of A1 and A2, adding J6 could promote the growth of ryegrass and increase biomass. The inoculation of J6 bacteria could promote the uptake of Pb and Cd in ryegrass. Under different concentrations of heavy metals, the content of Cd in the upper ryegrass was increased after the treatment with bacteria. The highest content was 27%, and the content of Pb and Cd in the underground part increased by 17%~64% and 5%~23%, respectively. After adding the EDTA, the Pb concentration of the plant root increased by 40% and 60% in A5 and A6, respectively. The inoculation of J6 could significantly reduce the total amount of Pb and Cd in rhizosphere soils; meanwhile, J6 could effectively improve the available content of Pb and Cd. Additionally, the inoculation of J6 bacteria could also improve the transport coefficient of Cd in ryegrass. In general, the inoculation of J6 bacteria could promote the uptake of Cd and Pb by ryegrass in soil polluted by heavy metals to a greater extent than did the addition of chelator EDTA. |
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