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| The antibiotic resistance of microorganisms and the functional diversity of microbial communities in the aquaculture environments of Honghu Lake, China |
| Received:October 27, 2017 |
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| KeyWord:Honghu Lake;aquaculture;microbial;antibiotic resistance;Biolog |
| Author Name | Affiliation | E-mail | | GUAN Chuan | School of Environmental Studies, China University of Geosciences, Wuhan 430074 | | | TONG Lei | School of Environmental Studies, China University of Geosciences, Wuhan 430074 | tonglei@cug.edu.cn | | QIN Li-ting | School of Environmental Studies, China University of Geosciences, Wuhan 430074 | | | LIU Hui | School of Environmental Studies, China University of Geosciences, Wuhan 430074 | |
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| Abstract: |
| For a better understanding of the influence of antibiotics on microbes in aquaculture environments, samples were collected from groundwater, lake water, and fish ponds in the aquaculture areas of Honghu Lake. The number of bacteria, fungi, and actinomycetes, and their antibiotic resistances to sulfonamides in different water samples were studied. The correlations of the number of antibiotic resistant microorganisms, and the proportions of resistant population with the concentration of sulfonamides were analyzed. The functional diversity of the microbial communities was studied by Biolog analysis. The results showed that the number of microorganisms in different water samples were as follows:fish ponds > lake water > ground water; The microbial numbers in lake water and fish ponds had larger differences due to the influence of human activities. Meanwhile, the proportions of resistant microorganisms were in the order:lake water > fish ponds > ground water. A significant positive correlation was observed between the number of resistant bacteria and fungi, and the concentration of pyridine sulfonamide and sulfamethazine in surface water, although no such significant correlations were found in the case of groundwater. Furthermore,the proportions of resistant bacteria and actinomycetes in surface water were significantly correlated with the concentrations of pyridine sulfonamide. The results of Biolog analyses showed that the metabolic activity and the carbon source utilization of microbial communities in surface water were higher than that of ground water, which indicated the rich microbial species for the different carbon source utilizations in lake water and fish ponds. In summary, the number of microorganisms, their antibiotic resistances, and the functional diversity of the microbial community in various water environments were different, and the differences were related to human activities and antibiotic concentrations. |
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