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| Effect of various organic acids on Agrobacterium species colonization in the rhizosphere of Chinese flowering cabbages |
| Received:February 05, 2018 |
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| KeyWord:Agrobacterium species;Chinese flowering cabbages;colonization;phosphorus;potassium |
| Author Name | Affiliation | E-mail | | LÜ Yao | Key Laboratory of Environmental Pollution and Health of Guangdong Province, School of Environment, Jinan University, Guangzhou 510632, China | | | WANG Li-li | Key Laboratory of Environmental Pollution and Health of Guangdong Province, School of Environment, Jinan University, Guangzhou 510632, China | | | XU Zhi-min | Key Laboratory of Environmental Pollution and Health of Guangdong Province, School of Environment, Jinan University, Guangzhou 510632, China | | | LI Qu-sheng | Key Laboratory of Environmental Pollution and Health of Guangdong Province, School of Environment, Jinan University, Guangzhou 510632, China | liqusheng@21cn.com | | LIN Xin | Key Laboratory of Environmental Pollution and Health of Guangdong Province, School of Environment, Jinan University, Guangzhou 510632, China | | | ZHOU Ting | Key Laboratory of Environmental Pollution and Health of Guangdong Province, School of Environment, Jinan University, Guangzhou 510632, China | | | YE Han-jie | Key Laboratory of Environmental Pollution and Health of Guangdong Province, School of Environment, Jinan University, Guangzhou 510632, China | | | GAO Qiong | Key Laboratory of Environmental Pollution and Health of Guangdong Province, School of Environment, Jinan University, Guangzhou 510632, China | |
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| Abstract: |
| This bacterial strain, which has potassium-solubilizing ability, was isolated from farmland soils. T29 was identified as Agrobacterium species based on morphological, physiological, and biochemical characteristics and 16S rDNA sequence analysis. Shake flask experiments were used to assess the ability of T29 to mobilize insoluble phosphorus and potassium. Then, the colonization of the rhizosphere by T29, induced by various organic acids and amino acids, was investigated via chemotaxis, swarming, in vitro, and pot experiments. In the shake flask experiment, the mobilized phosphorus and potassium contents were 15.53 mg·L-1 and 2.78 mg·L-1, respectively, which were 1.42 times and 1.54 times those of the control groups. In the chemotaxis and swarming experiments, the number of colonies and the diameter of strain T29 grown in culture medium with gluconic acid were 3.65×106 cfu·mL-1 and 2.18 cm, respectively. In the in vitro experiments, the colony number on the root surface was 2.98×106 cfu·g-1 fresh root weight and was 29.1% higher than that of the control groups. In the pot experiments, compared with the control groups, available phosphorus and potassium in the rhizosphere soil increased by 37.5% and 52.8%, respectively. The phosphorus and potassium concentrations in roots did not change significantly (P>0.05), but those in shoots increased significantly. Compared with the control groups, plant biomass and height increased significantly (P<0.05). These results suggested various organic acids and amino acids influence T29 colonization differently. Gluconic acid preferably strengthened the colonization process of T29 in the rhizosphere of Chinese flowering cabbages, thereby mobilizing more soil phosphorus and potassium and promoting plant growth. Our study provides a possible method for reducing the use of chemical fertilizers. |
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