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| Identification of 15N-DNA enrichment sites in DNA-SIP to reveal functional genes by qPCR from sugarcanesoybean intercropping soil |
| Received:March 15, 2018 |
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| KeyWord:sugarcane-soybean intercropping;stable isotope probing;real-time PCR;functional genes |
| Author Name | Affiliation | E-mail | | GOU Yong-gang | Institute of Tropical and Subtropical Ecology, South China Agricultural University, Guangzhou 510642, China
Key Laboratory of Agroenvironment in the Tropics, Ministry of Agriculture and Rural Affairs, Guangzhou 510642, China
Department of Ecology, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China | | | YU Ling-ling | Institute of Tropical and Subtropical Ecology, South China Agricultural University, Guangzhou 510642, China
Key Laboratory of Agroenvironment in the Tropics, Ministry of Agriculture and Rural Affairs, Guangzhou 510642, China
Department of Ecology, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China | | | XU Xia | Institute of Tropical and Subtropical Ecology, South China Agricultural University, Guangzhou 510642, China
Key Laboratory of Agroenvironment in the Tropics, Ministry of Agriculture and Rural Affairs, Guangzhou 510642, China
Department of Ecology, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China | | | WANG Jian-wu | Institute of Tropical and Subtropical Ecology, South China Agricultural University, Guangzhou 510642, China
Key Laboratory of Agroenvironment in the Tropics, Ministry of Agriculture and Rural Affairs, Guangzhou 510642, China
Department of Ecology, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China | wangjw@scau.edu.cn |
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| Abstract: |
| Using DNA stable isotope probes(DNA-SIPs) is a reliable, new technique for studying the nitrogen cycle. To identify indicator function genes of 15N-DNA enrichment in ultra-high-speed centrifugation in a sugarcane-soybean intercropping system for DNA-SIPs, the relative abundance distributions of six nitrogen cycling functional genes in the DNA of different buoyant density centrifugation fluids were detected via real-time PCR(qPCR). Through the analysis of the relative abundance of nitrogen cycling functional genes, the gene abundance peaks of the nifH and amoA genes in sugarcane-soybean intercropping and soybean monoculture were shifted in the 15N marker group and the control group, the gene abundance peaks of chiA were only shifted in soybean monocropping mode, and the abundance peaks of nirS, nirK, and nosZ did not shift with either planting pattern. The results showed that the nifH and amoA genes could be used as indicator genes to effectively identify DNA-SIP technology 15N-DNA positions in sugarcane-soybean intercropping systems. |
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