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Microbial community structure in the roots of three kinds of plants in integrated vertical flow constructed wetlands
Received:February 27, 2018  
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KeyWord:constructed wetlands;microbial communities;Chao1 index;up-flow cell;down-flow cell
Author NameAffiliationE-mail
JIANG Xu-yao Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China  
JI Xi-yan Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China  
HUANG De-ying Department of Chemistry, Fudan University, Shanghai 200433, China huangdeying@fudan.edu.cn 
ZHANG Ji-biao Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China  
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Abstract:
      The aim of this study is to analyze the microbial community structure in the roots of Acorus calamus L., Canna indica L., and Scirpus validus Vahl wetlands using microbial diversity sequencing technology. The abundance of root microorganisms in autumn were higher than that in summer, followed by that in winter. There was no obvious difference in microorganism relative abundances between up-flow and down-flow cells in Scirpus validus Vahl wetland. Down-flow cells of Acorus calamus L. and Canna indica L. wetlands showed higher abundances of root microorganisms than those of up-flow cells, except for Canna indica L. wetland in October. The microbial community members were similar in different cells. Furthermore, the similarity between up-flow cells was higher than that between down-flow cells. At the genus level, Nitrospira and Candidatus Nitrosophaera, which are nitrifying bacteria, were detected, and Dechloromonas, Flavobacterium, and Pseudomonas, as denitrifying bacteria, were detected. Except for the down-flow cells of Canna indica L.wetland, the relative abundances of nitrifying bacteria in Acorus calamus L. wetland and Scirpus validus Vahl wetland in autumn were higher than those in summer. This study could provide some help for improving the microbial community structure of constructed wetlands.