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| Dynamic changes in culturable Bacillus-like species populations in the process of sugar-refinery filtering mud composting fermentation to produce bio-organic fertilizers |
| Received:January 16, 2018 |
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| KeyWord:sugar-refinery filtering muds;composting fermentation;Bacillus-like species;dynamic changes |
| Author Name | Affiliation | | LIU Bo | Agricultural Bio-resoarces Institute, Fujian Academy of Agriculture Sciences, Fuzhou 350002, China | | CHEN Qian-qian | Agricultural Bio-resoarces Institute, Fujian Academy of Agriculture Sciences, Fuzhou 350002, China | | WANG Jie-ping | Agricultural Bio-resoarces Institute, Fujian Academy of Agriculture Sciences, Fuzhou 350002, China | | CHEN Zheng | Agricultural Bio-resoarces Institute, Fujian Academy of Agriculture Sciences, Fuzhou 350002, China | | DENG Wen-qiong | Agricultural Bio-resoarces Institute, Fujian Academy of Agriculture Sciences, Fuzhou 350002, China | | LIU Xin | Agricultural Bio-resoarces Institute, Fujian Academy of Agriculture Sciences, Fuzhou 350002, China | | XIAO Qian | Yuyuan Biological Technology Co., Ltd, Changsha 410001, China | | TANG Guo-liang | Yuyuan Biological Technology Co., Ltd, Changsha 410001, China |
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| Abstract: |
| The aim of this study is to determine dynamic changes in Bacillus-like species population in the process of sugar-refinery filtering mud composting fermentation to produce bio-organic fertilizers. The Bacillus-like species in the samples at different composting and processing stages were isolated and identified to analyze the dynamic changes in their population size and structure during the process, and to reveal their relationship with the indigenous Bacillus-like species. The results indicated that the Bacillus-like species were enriched in the process of sugar-refinery filtering mud composting fermentation. A total of 64 strains, belonging to 27 species within 7 genera, were isolated from 6 samples at different composting and processing stages. Among them, the genus Bacillus, including 16 species, was the absolutely dominant population. Furthermore, 6 species were Bacillus-like new record species in China, including B. glycinifermentans(FJAT-47935, FJAT-47944, and FJAT-47907), B. hisashii(FJAT-47926), Bhargavaea cecembensis(FJAT-47948), Lysinibacillus acetophenoni (FJAT-47912, FJAT-47934, and FJAT-47919), Ornithinibacillus bavariensis(FJAT-47915), and Ornithinibacillus scapharcae(FJAT-47951). There were significant differences in the Bacillus-like species population size and structure during different composting and processing stages, with a size variation range from -80% to +100% and a species variety rate from 62% to 84%. The content of the Bacilluslike species populations was 1.14×106, 2.37×106 cfu·g-1 and 3.76×105 cfu·g-1 at the early(1 d), middle(30 d), and later(60 d) stages, respectively, of composting fermentation, and that was 4.3×105 cfu·g-1 after the production process. The indigenous Bacillus-like species had less influence on composting fermentation of the sugar-refinery filtering muds; the introduced Bacillus-like species during composting fermentation were mainly brought by the sugar-refinery filtering muds themselves. Therefore, the self-brought Bacillus-like species could fully develop their actions during the sugar-refinery filtering mud composting fermentation, although any additional Bacillus-like inoculation agent was not used. In conclusion, the Bacillus-like species are rich and their population size and structure are significantly different in the process of sugar-refinery filtering mud composting and processing. |
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