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| Isolation and degradation characteristics of haloalkaliphilic high molecular-weight polycyclic aromatic hydrocarbon-degrading bacteria |
| Received:August 10, 2018 |
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| KeyWord:high molecular weight PAHs;haloalkaliphilic bacteria;microbial degradation;pyrene;benzo(a) pyrene |
| Author Name | Affiliation | | FAN Rui-juan | College of Biological Science & Engineering, North Minzu University, Ningxia Key Laboratory of Microbial Resources Development and Applications in Special Environment, Key Laboratory of Fermentation Engineering and Biotechnology of State Ethnic Affairs Commission, Yinchuan 750021, China | | LIU Ya-qin | College of Biological Science & Engineering, North Minzu University, Ningxia Key Laboratory of Microbial Resources Development and Applications in Special Environment, Key Laboratory of Fermentation Engineering and Biotechnology of State Ethnic Affairs Commission, Yinchuan 750021, China | | ZHANG Xiu | College of Biological Science & Engineering, North Minzu University, Ningxia Key Laboratory of Microbial Resources Development and Applications in Special Environment, Key Laboratory of Fermentation Engineering and Biotechnology of State Ethnic Affairs Commission, Yinchuan 750021, China |
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| Abstract: |
| To obtain the haloalkaliphilic high molecular-weight polycyclic aromatic hydrocarbon (PAH) -degrading bacteria and study their degradation characteristics, six haloalkaliphilic strains (SYP-1, SYP-2, SYP-3, SYP-4, SYP-5, and SYP-11) were isolated from oilpolluted soil in the Yanchang oilfield with pyrene as the sole carbon and energy source in a mineral salt medium with a salinity of 5% and a pH of 8.6. Based on morphological observations, physiochemical characteristics, and 16S rRNA sequences analysis, SYP-1 was identified as Delftia sp.; SYP-2, SYP-4, and SYP-11 were identified as Marinobacter sp.; and SYP-3 and SYP-5 were identified as Bacillus sp. A preliminary degradation ability experiment showed that 42.3%~68.8% of pyrene with an initial concentration of 50 mg·L-1 was degraded and 27.0%~49.4% of benzo (a) pyrene with an initial concentration of 5 mg·L-1 was degraded by the six degrading strains in 7 days. Four strains (SYP-2, SYP-3, SYP-4, and SYP-11) with high degradation ability for pyrene and benzo[a]pyrene were selected to analyze the effects of different salinity and pH on the degradation efficiency of pyrene. In the range of 0~15% salinity or pH range of 5~10, all four strains showed efficient degradation ability of pyrene. Thus, the isolated strains have high salinity and alkali resistance, and great potential to degrade high molecular weight PAHs above four rings. |
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