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| Isolation, identification, and degrading properties of allelochemicals-degrading bacteria of peanuts |
| Received:October 30, 2018 |
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| KeyWord:degrading bacteria;allelochemicals;degrading property;peanuts |
| Author Name | Affiliation | E-mail | | QIAN Na | College of Environmental Science and Technology, Yangzhou University, Yangzhou 225127, China | | | WANG Xiao-bing | College of Environmental Science and Technology, Yangzhou University, Yangzhou 225127, China
Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Yangzhou University, Yangzhou 225127, China | xbwangxl@sina.com.cn | | WANG Xiao-li | College of Environmental Science and Technology, Yangzhou University, Yangzhou 225127, China | | | FENG Ke | College of Environmental Science and Technology, Yangzhou University, Yangzhou 225127, China
Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Yangzhou University, Yangzhou 225127, China | | | CHEN Dun | College of Environmental Science and Technology, Yangzhou University, Yangzhou 225127, China | | | SU Jin-cheng | College of Environmental Science and Technology, Yangzhou University, Yangzhou 225127, China | | | CUI Meng-han | College of Environmental Science and Technology, Yangzhou University, Yangzhou 225127, China | |
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| Abstract: |
| Allelopathy is one of the most important factors inducing peanut replant disease. Thus, screening out highly efficient degrading bacteria is an essential means to inhibit peanut allelopathy. Benzoic acid has been considered one of the main allelochemicals in peanut root-exudates. Using benzoic acid as the sole carbon source, degrading bacteria were screened from the rhizosphere soil of healthy peanut plants, in a long-term experiment of peanut monoculture, through enrichment culture and dilution-plate methods. The degrading bacteria were identified by morphological characteristics and 16S rDNA homology comparison. The degrading properties of bacteria were analyzed by single factor tests. Two allelochemical-degrading bacteria were isolated and named strain HJ-2 and strain HJ-3, which belonged to Achromobacter xylosoxidans and Pseudomonas nitroreducens respectively. The appropriate degrading conditions for HJ-2 were pH 6.0~9.0, 30~45℃, and 0~6% NaCl. For strain HJ-3, the appropriate degrading conditions were pH 6.0~8.0, 30~35℃, and 0~4% NaCl. Besides the high efficiency of benzoic acid degradation, both strains degraded some other allelochemicals, including acetophenone, stearic acid, palmitic acid, lactic acid, 3,5-dimethylbenzaldehyde, and glycerol. The HJ-2 and HJ-3 strains both had broad-spectrum degradation characteristics. However, due to its stronger environment adaption, the HJ-2 strain provided theoretical significance for the governance of peanut replant disease in the future. |
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