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| Specificity of humic acid-like(HAL)substance from Trichoderma reesei-inoculated corn straw |
| Received:April 02, 2019 |
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| KeyWord:humification;corn straw;Trichoderma reesei;specificity of humic substance;humic acid-like(HAL) |
| Author Name | Affiliation | E-mail | | ZHANG Yi-feng | College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China | | | DOU Sen | College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China | dousen1959@126.com | | YE Shu-fen | College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China | | | ZHANG Dan-dan | College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China | |
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| Abstract: |
| To investigate the structural features of humic acid-like (HAL)substance from corn straw during the humification process of Trichoderma reesei, the alkaline extract of the culture product (Humic-like substances, HLS)was obtained. The extract demonstrated similar specificities to soil HA. Corn straw composting was simulated using solid culture. The humification process of corn straw was simulated for a period of 30 d using two treatments: (1)adding T. reesei and (2)uninoculated control after sterilization (CK). Samples were collected at 0, 15 d, and 30 d for observation. The HAL substance formed by T. reesei using corn straw were analyzed using elemental composition, infrared spectroscopy, and differential thermal techniques, and the specificity was determined from comparison with soil HA. The results showed that, compared with CK, inoculation with T. reesei significantly changed the structural characteristics of HAL substances and the ratio of aromatics/aliphatics during humification, which is in accordance with polyphenol theory. T. reesei concentrated the elemental structure of the HAL substance at O/C=0.528 and H/C=1.322 on day 30, and the difference between HAL and HA was reduced by 71.08% and 49.25%, with the HAL displaying similarity with the structural characteristics of HA. In general, T. reesei can effectively promote the conversion of corn straw to humus through cellulose and lignin decomposition and structural changes in HAL substances. The differences between the structural characteristics of soil HA and HAL from T. reesei culture decrease significantly during the humification process. |
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