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| Effect of cadmium stress on non-protein thiols in the seedlings of high- and low-cadmium-accumulating rice cultivars |
| Received:September 12, 2019 |
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| KeyWord:cadmium stress;rice;non-protein thiols compounds;reduced glutathione;phytochelatins;glutathione S-transferase |
| Author Name | Affiliation | E-mail | | LI Dong-qin | Life Science and Technology School, Lingnan Normal University, Zhanjiang 524048, China
Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, China | | | WANG Li-li | Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, China | | | LI Zhi-ming | Guangzhou Ecological Research Co., Ltd, Guangzhou 510640, China | | | LI Hua-shou | Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, China | | | CHEN Gui-kui | Key Laboratory of Agro-Environment in the Tropics, Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou 510642, China | guikuichen@scau.edu.cn |
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| Abstract: |
| In order to better understand the influence of cadmium(Cd)stress on non-protein thiols(NPT)compounds in the seedlings of high- and low-Cd-accumulating rice cultivars, a pot experiment was conducted. In the experiment, 50 μmol·L-1 Cd2+ was set up, the peroxidation degree and NPT content were to be monitored regularly at before treatment(0 d), at 1st, 3rd, 5th and 7th d after treatment. The results showed that the contents of hydrogen peroxide(H2O2)and thiobarbituric acid reactive substances(TBARS)in the two cultivars induced by Cd showed an increasing trend over time, and the extents of variation for H2O2 and TBARS content in You I2009(low-Cd-accumulating rice cultivar)were greater than that in Xinrongyou 2045(high-Cd-accumulating rice cultivar), proving with data that the extents of variation for H2O2 and TBARS contents in Xinrongyou 2045 cultivar were 45% and 74% for shoots, and 1.14 folds and 61% for roots, respectively, while they were 78% and 1.20 folds for shoots, and 1.32 folds and 64% for roots in You I2009 cultivar. It indicated that You I2009 suffered more oxidative stress and membrane lipid peroxidation than Xinrongyou 2045. An increasing pattern in reduced glutathione(GSH) content of Xinrongyou 2045 were induced by Cd stress over time while for You I2009, the GSH contents increased first and then decreased. The NPT content, phytochelatins(PCs), and glutathione S-transferase(GST)in the two cultivars increased first and then decreased over time under Cd stress, and the NPT contents and GST activities of Xinrongyou 2045 were higher than that in You I2009. These results indicate that high-Cd-accumulating rice cultivar can eliminate reactive oxygen species by synthesizing more sulfhydryl nonprotein compounds chelating with cadmium. |
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