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| Use of transgenic zebrafish in the study of cadmium immunotoxicity |
| Received:June 29, 2019 |
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| KeyWord:cadmium;transgenic;zebrafish;immune toxicity |
| Author Name | Affiliation | E-mail | | ZHAO Shuang | School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384, China | | | DUAN Xin-yue | School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384, China | | | WANG Jiao | School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384, China | | | FENG Yi-fan | School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384, China | | | WANG Yu-di | School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384, China | | | GONG Zhi-yuan | Department of Biological Sciences, National University of Singapore, Singapore 117543 | | | DUAN Zheng-hua | School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384, China | duanzhenghua@mail.nankai.edu.cn |
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| Abstract: |
| To use transgenic zebrafish in research on the immunotoxicity of environmental pollutants, transgenic zebrafish Tg(lysC:DsRed2)-Lyz fish was modelled, and zebrafish embryo development technology and quantitative real-time RT-PCR(Reverse Transcription-Polymerase Chain Reaction)technology were carried out in this study. The immunotoxicity of cadmium and its toxic mechanism were discussed. The results showed that the incubation of transgenic zebrafish embryos could be significantly inhibited by 0.01 μmol·L-1 CdCl2 (P=0.013). When the exposure concentration increased to 0.1 μmol·L-1, 24 h blood flow disorder and 52 h hatch inhibition were both greatly enhanced in the fish embryo, and these toxic effects were significantly dose dependent. The level of neutrophils that were labelled with lysozyme(lysozyme C, lyz)in zebrafish larvae was increased upon 0.01 μmol·L-1 CdCl2 exposure, and the neutrophils tended to aggregate in the fish liver. The mechanism of immunotoxicity was related to the induction of the expression of the metallothionein related gene (dMT)and inflammation related genes(serp, tnf), and the inhibition of the expression of cancer related genes(ccgn1, chk2, p53). Therefore, the transgenic zebrafish Tg(lysC:DsRed2) -Lyz fish could play important roles in the evaluation of the immunotoxicity of heavy metal pollutants. |
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