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| Evaluation of combined toxicity of triazine pesticide contaminants against Chlorella pyrenoidosa |
| Received:October 16, 2019 |
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| KeyWord:triazine pesticides;Chlorella pyrenoidosa;combined toxicity;chlorophyll a;three-dimensional isobologram |
| Author Name | Affiliation | E-mail | | WANG Tao | Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China | | | BAN Long-ke | Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China | | | ZHANG Jin | Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China | ginnzy@163.com | | BIAN Zhi-qiang | Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China | | | PAN Fa-kang | Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province, College of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China | |
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| Abstract: |
| In order to explore the toxicity of pesticide mixtures to non-target organisms, three triazine pesticides:metamitron(Met), bladex (Bla), and terbumeton(Ter)being as research objects and Chlorella pyrenoidosa(C. pyrenoidosa)as a test organism, direct equipartition ray and uniform design ray procedures were used to design binary and ternary mixture systems, respectively. The time-concentration-effect curves of triazine pesticide contaminants on C. pyrenoidosa were determined by using the microplate toxicity analysis method. Concentration addition (CA)was used as a standard reference model to analyze the toxicity interaction within mixture systems. Two-dimensional isobologram and three-dimensional isobologram were applied to characterize combined toxicity interactions of mixtures. The change of chlorophyll a content in C. pyrenoidosa was analyzed to further evaluate the toxic effects of pesticides and their mixtures on C. pyrenoidosa. The results show that the Logit function can effectively describe the toxicity of pesticides and their mixtures toward C. pyrenoidosa, the toxicity order of the three triazines is as follows:Met > Bla > Ter. Based on the results predicted by CA model, toxicity interaction within binary mixtures changes from additive action to synergism, two-dimensional isobolograms indicate that the three binary mixture systems all show strong synergism at the median effective concentration. Five rays of ternary mixture systems all exhibit additive action which remain unchanged with the lengthening of exposure time. The results predicted by CA model are the same as those of three-dimensional isobolograms. The reduction rate of chlorophyll a content is consistent with the changing trends of the pesticides' concentration-response curves. |
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