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| Response of amino acids in ryegrass root exudates to polycyclic aromatic hydrocarbon stress |
| Received:April 13, 2020 |
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| KeyWord:ryegrass;root exudates;amino acid;polycyclic aromatic hydrocarbons(PAHs) |
| Author Name | Affiliation | E-mail | | HU Fang-yu | Qilu University of Technology(Shandong Academy of Sciences), Ecology Institute of Shandong Academy of Sciences, Shandong Provincial Key Laboratory of Applied Microbiology, Jinan 250103, China | | | MENG Fan-bo | Jinan Environmental Research Institute, Jinan 250102, China | | | ZHANG Wen | Qilu University of Technology(Shandong Academy of Sciences), Ecology Institute of Shandong Academy of Sciences, Shandong Provincial Key Laboratory of Applied Microbiology, Jinan 250103, China | zw-sunshine@163.com | | GAO Yong-chao | Qilu University of Technology(Shandong Academy of Sciences), Ecology Institute of Shandong Academy of Sciences, Shandong Provincial Key Laboratory of Applied Microbiology, Jinan 250103, China | | | ZHENG Li-wen | Qilu University of Technology(Shandong Academy of Sciences), Ecology Institute of Shandong Academy of Sciences, Shandong Provincial Key Laboratory of Applied Microbiology, Jinan 250103, China | |
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| Abstract: |
| In this study, a hydroponics experiment was conducted to investigate the effects of different concentrations of phenanthrene (PHE), pyrene(PYR), and benzo(a)pyrene(BAP)on the amino acids in ryegrass root exudates. A total of(84.2±10.3)μg·g-1 of amino acids in ryegrass root exudates was identified in the control group. In total, ten amino acids were detected, of which valine and glutamic acid accounted for 43% and 24%, respectively. The biomass of ryegrass increased to different extents after exposure to polycyclic aromatic hydrocarbons(PAHs), which showed different effects on the total concentration and composition of the amino acids. The total concentration of amino acids in the root exudates decreased in PHE and BAP-L groups and an increased inhibition effect was observed with the increase in PHE concentration. Other treatments did not result in significant differences. The exudation of tyrosine was not detected under PHE stress while the exudation of valine was inhibited. At the same time, the exudation of glutamic acid was inhibited by PHE-H stress. However, the exudation of isoleucine and leucine were promoted by PYR stress. In addition, the exudation of methionine was promoted by PYR-H stress while the exudation of valine was inhibited and that of histidine was stopped by PYR-L stress. The exudation of isoleucine and leucine were initiated by BAP stress, which stopped the exudation of tyrosine and inhibited the exudation of valine. Furthermore, the exudation of methionine was promoted by BAP-H stress and that of histidine was stopped by BAP-L stress. Exploration of the effect of PAHs on amino acids in ryegrass root exudation can provide insights into the mechanism of phytoremediation in contaminated soils. |
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