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| Effects of fertilization regimes on the metabolic diversity of microbial carbon sources in a maize field of fluvoaquic soil in North China |
| Received:May 07, 2020 |
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| KeyWord:fertilization regimes;soil microbial community;functional diversity;Biolog EcoPlate;maize field |
| Author Name | Affiliation | | LIU Hong-mei | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | AN Ke-rui | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | WANG Hui | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | ZHANG Si-yu | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | ZHAO Jian-ning | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | YANG Dian-lin | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | ZHANG Gui-long | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China |
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| Abstract: |
| To reveal the effects of long-term fertilization regimes on the metabolic diversity of the soil microbial community of a maize field in North China in order to provide a theoretical basis and data support for sustainable fertilization management in North China. This study was conducted in the Wuqing Experimental Station of the Agro-Environmental Protection Institute of the Ministry of Agriculture and Rural Affairs. The field experiment included six treatments, no fertilizer(A0), organic fertilizer(A1), reduced nitrogen fertilizer plus organic fertilizer(A2), normal chemical fertilizer plus organic fertilizer(A3), increased nitrogen fertilizer plus organic fertilizer(A4), and chemical fertilizer(A5). Soil samples were separately collected from the 0~20 cm soil layer. A Biolog EcoPlate was applied to test the changes in the soil microbial diversity under different fertilization treatments. The results showed that compared with the A0 treatment, the A1, A2, A3, A4, and A5 treatments significantly increased the contents of total nitrogen and nitrate nitrogen and significantly decreased the soil carbon/nitrogen ratio and pH. The application of organic fertilizer treatments(A1, A2, A3, and A4)significantly increased the organic carbon and microbial biomass nitrogen content. The average well color development values of the soil microbial communities in culture for 96 h were as follows:A2 > A3 > A4 > A5 > A1 > A0. The Shannon index of the A2 treatment was significantly higher than that of the other fertilization treatments. The results of the principal component analysis showed that there were significant differences in the microbial utilization of carbon in the different fertilization treatments. The A2 and A3 treatments were concentrated in the positive direction of the first principal component with score coefficients ranging from 1.125 to 1.473, and had similar carbon utilization. Conversely, the A0, A1, A4, and A5 treatments were concentrated in the negative direction of the first principal component, and the score coefficients ranged from -1.157 to -0.167. The carbon sources mostly used by soil microbes were carbohydrates, carboxylic acids, amino acids, and polymers. The correlation analysis showed that the soil carbon/nitrogen ratio, ammonium nitrogen, nitrate nitrogen, pH, and microbial biomass nitrogen were the main factors affecting the metabolic diversity of the soil microbial carbon sources. The A2 treatment improved the activity and functional diversity of the soil microbial community. |
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