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| Study of survival conditions and growth of petroleum hydrocarbon-degrading bacteria in petroleumcontaminated soil |
| Received:May 13, 2020 |
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| KeyWord:hydrocarbon-degrading bacteria;petroleum-contaminated soil;bioaugmentation;community structure;inoculation amount;moisture content |
| Author Name | Affiliation | E-mail | | WANG Di | Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resources, Environment and Ecology, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China | | | MA Chuang | Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resources, Environment and Ecology, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China | | | GAO Huan | Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resources, Environment and Ecology, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China | | | LIU Heng | Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resources, Environment and Ecology, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China | | | XU Hui-ning | Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resources, Environment and Ecology, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China | | | WU Man-li | Shaanxi Key Laboratory of Environmental Engineering, Key Laboratory of Northwest Water Resources, Environment and Ecology, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China | 447005853@qq.com |
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| Abstract: |
| Bioremediation of petroleum-contaminated soil by inoculating hydrocarbon-degrading microorganisms is widely applied presently. However, there are few studies on the growth and survival of hydrocarbon-degrading microorganisms and their effects on petroleum hydrocarbon degradation. In this study, the proliferation and survival conditions of inoculating strains, as well as their effects on petroleum degradation, were determined by laboratory simulation of remediation. The results showed that the compositions of petroleumdegrading bacterial flora mainly included the Proteobacteria phylum(99.75%), Gamma-proteobacteria class(99.49%), Pseudomonadales order(99.36%), Moraxellaceae family(87.33%)Acinetobacter sp.(87.32%), and Pseudomonadaceae family(12.04%)Pseudomonas sp. (12.00%). When the soil moisture content and the inoculation amount of degrading strains were 5.4% and 108 cfu·g-1 respectively, the removal efficiency of total petroleum hydrocarbons was 10.61%; the removal efficiency increased to 18.67% when the soil moisture content and the bacteria inoculation amount were 15.0% and 107 cfu·g-1, respectively. After 7 days of inoculation, the relative abundance of the Proteobacteria phylum increased from 28.22% to 57.98%~66.35%; Acinetobacter genus increased from 0.04% to 25.86%~30.25%; and Pseudomonas genus increased from 0.26% to 5.03%~30.87%. The results indicated that the strains proliferated rapidly after 7 days of inoculation under various soil moisture contents. Inoculation of exogenous bacteria changed the soil bacterial community structures and reduced the alpha diversity significantly. The removal of the contaminants not only depends on the specific function of some dominant bacteria but also requires the synergetic metabolism of soil flora. |
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