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| Effects of copper on tissues of juvenile Anodonta woodiana |
| Received:November 30, 2020 |
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| KeyWord:Anodonta woodiana;copper;tissue section;toxicological effect;fishery ecological environment |
| Author Name | Affiliation | E-mail | | LIU Kai | Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China | | | CHEN Xiu-bao | Key Laboratory of Fishery Ecological Environment Assessment and Research Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | LIU Hong-bo | Key Laboratory of Fishery Ecological Environment Assessment and Research Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | JIANG Tao | Key Laboratory of Fishery Ecological Environment Assessment and Research Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | | | YANG Jian | Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China
Key Laboratory of Fishery Ecological Environment Assessment and Research Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China | jiany@ffrc.cn |
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| Abstract: |
| To explore the toxic effects of Cu on the gill, digestive gland, mantle, and foot tissues of juvenile Chinese pond mussel（Anodonta woodiana）, the Cu concentration gradients were set as 2.0, 1.0, 0.1, 0.01, and 0.005 mg·L-1 according to the corresponding values of the 96 h EC50 and the Cu limit in China's Fishery Water Quality Standard（GB 11607—1989）. The results showed that 0.005 mg·L-1 of Cu did not cause clear tissue damage. After 96 h of exposure, the gills of the 0.01 mg·L-1 group showed a significant increase in pigment cells and cell vacuolization. In the gills of the mussels exposed to 0.1 mg·L-1 of Cu, macrophages began to increase and cilia fell off. In the group exposed to 2.0 mg·L-1 of Cu, gill cell necrosis, filament atrophy, and connective tissue erosion were observed. Cell vacuolation and epithelial damage of the foot occurred in the mussels exposed to 1.0 mg·L-1 of Cu, and this effect was clearly aggravated when exposed to 2.0 mg·L-1. Significant increases in the pigment cells and macrophages of the mantle were found for the mussels exposed to 0.01 mg·L-1 of Cu, whereas the epithelial layer was damaged at 2.0 mg·L-1 exposure. The digestive glands presented tubular epithelial cell deformation, an enlarged lymphatic area, and connective tissue atrophy at 0.1 mg·L-1 exposure. The results suggested that the gills were the most sensitive tissue of the mussel to Cu toxicity, and thus could be used as a target tissue for Cu contamination monitoring and toxicity assessment of the ecological environment of freshwater fisheries. |
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