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| Effect of ciprofloxacin on the degradation of 4-chloro-1-nitrobenzene by Comamonas testosterone CT1 |
| Received:January 12, 2021 |
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| KeyWord:ciprofloxacin;4-chloro-1-nitrobenzene;Comamonas testosterone;biodegradation;superoxide dismutase |
| Author Name | Affiliation | E-mail | | LIU Yonghao | Key Laboratory of Straw Biology and Higher Value Application, Ministry of Education, College of Life Science, Jilin Agricultural University, Changchun 130118, China | | | XU Miao | Key Laboratory of Straw Biology and Higher Value Application, Ministry of Education, College of Life Science, Jilin Agricultural University, Changchun 130118, China | | | WU Ming | Key Laboratory of Straw Biology and Higher Value Application, Ministry of Education, College of Life Science, Jilin Agricultural University, Changchun 130118, China | | | LUO Jun | Key Laboratory of Straw Biology and Higher Value Application, Ministry of Education, College of Life Science, Jilin Agricultural University, Changchun 130118, China | | | SHANG Jiaqi | Key Laboratory of Straw Biology and Higher Value Application, Ministry of Education, College of Life Science, Jilin Agricultural University, Changchun 130118, China | | | GUO Liquan | Key Laboratory of Straw Biology and Higher Value Application, Ministry of Education, College of Life Science, Jilin Agricultural University, Changchun 130118, China
National Environmental Protection Wetland Ecology and Vegetation Restoration Key Laboratory, School of Environment, Northeast Normal University, Changchun 130024, China | guolq948@nenu.edu.cn |
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| Abstract: |
| To investigate the effect of antibiotics on the biodegradation of 4-chloro-1-nitrobenzene(4-CNB) in the environment, nine bacterial strains that can efficiently degrade 4-CNB were selected. The minimum inhibitory concentrations(MICs) of the 18 common antibiotics to CT1 were studied using a drug sensitivity test. The results showed that the degradation efficiency of 4-CNB by Comamonas testosterone CT1 was the highest, with a degradation efficiency of 98.4%±0.2% at 27℃ for 9 h. Only six antibiotics had MICs, among which ciprofloxacin had the lowest(4 μg·mL-1) and streptomycin had the highest(64 μg·mL-1). The effects of different concentrations of ciprofloxacin on cell concentration and activity, and the activity of total superoxide dismutase(SOD) and degradation were studied using 4-CNB-ciprofloxacin co-culture. When low concentration ciprofloxacin(1/4 MIC) was added, the cell concentration and activity decreased significantly, and the degradation rate of 4-CNB decreased to 87.0%±3.3%. When the concentration of ciprofloxacin was higher than the MIC(4 MIC), the strain barely grew, and the degradation rate of 4-CNB was only 35.7%±3.9%. The addition of ciprofloxacin significantly decreased SOD activity and caused damage to the reactive oxygen species. Therefore, the decrease of SOD activity caused by ciprofloxacin might be one of the main reasons for the inhibition of 4-CNB biodegradation. |
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