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| Role of substrate concentrations in the removal of tetracycline resistance genes in anaerobic digestion of dairy manure |
| Received:January 11, 2021 |
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| KeyWord:anaerobic digestion;substrate concentration;dairy manure;tetracycline resistance genes(TRGs);microbial communities |
| Author Name | Affiliation | E-mail | | ZHU Wenbo | School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China | | | ZHANG Qiuping | School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China | | | XU Jifei | School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China
Inner Mongolia Key Laboratory of Environmental Pollution Control and Waste Resource Recycle, Inner Mongolia University, Hohhot 010021, China | Jifeixu@imu.edu.cn | | PANG Xiao-ke | School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China | | | LIU Jianguo | College of Energy and Power Engineering, Inner Mongolia University of Technology, Hohhot 010051, China | | | ZHAO Ji | School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China
1. School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China | |
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| Abstract: |
| This study investigated the changes in tetracycline resistance genes(TRGs) in thermophilic anaerobic digestion with different substrate concentrations of 100%, 75%, 50%, and 25%, and the changes in TRGs, physical and chemical factors, and microbial communities and their relationships were analyzed. The results showed that the biogas production rates were similar among four treatments, and the total biogas yields increased from 358.83 mL·g-1·d-1 to 400.89 mL·g-1·d-1 as substrate concentration decreased from 100% to 25%. After anaerobic digestion, the relative abundances of tetO, tetQ, and tetT decreased by 1.04%~87.06%, whereas those of tetC, tetG, tetW, and tetX increased by 1.32~34.99 times. Compared with 100% substrate concentration, relatively lower substrate concentrations had the higher relative abundances of target genes after anaerobic digestion, such as tetC, tetG, tetO, tetX, and intⅠ1. Differences in microbial communities were observed among the four treatments. The dominant phylum changed from Firmicutes to Proteobacteria after digestion with lower substrate concentrations(50% and 25%). The species and number of potential host bacteria of TRGs also altered with the changes in substrate concentrations. Redundancy analysis demonstrated that physicochemical parameters affected the changes in TRGs by affecting their potential host bacteria. In conclusion, a low concentration of substrate due to the intervention wastewater might benefit the proliferation of potential host bacteria, thereby increasing the risks of transmission of TRGs. |
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