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| Construction and evaluation of bacterial-fungal consortia for rice straw degradation |
| Received:January 14, 2021 |
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| KeyWord:rice straw;degradation rate;bacterial-fungal consortia;cellulase;enzyme activity;construction strategy;diversity effect |
| Author Name | Affiliation | E-mail | | MEI Xinlan | College of Resources and Environmental Sciences, Nanjing Agricultural University
Jiangsu Provincial Key Lab of Solid Organic Waste Utilization
Jiangsu Collaborative Innovation Center of Solid Organic Wastes
Educational Ministry Engineering Center of Resource-saving Fertilizers
National Engineering Research Center of Organic-based Fertilizers, Nanjing 210095, China | | | ZHENG Haiping | College of Resources and Environmental Sciences, Nanjing Agricultural University
Jiangsu Provincial Key Lab of Solid Organic Waste Utilization
Jiangsu Collaborative Innovation Center of Solid Organic Wastes
Educational Ministry Engineering Center of Resource-saving Fertilizers
National Engineering Research Center of Organic-based Fertilizers, Nanjing 210095, China | | | LI Shuixian | College of Resources and Environmental Sciences, Nanjing Agricultural University
Jiangsu Provincial Key Lab of Solid Organic Waste Utilization
Jiangsu Collaborative Innovation Center of Solid Organic Wastes
Educational Ministry Engineering Center of Resource-saving Fertilizers
National Engineering Research Center of Organic-based Fertilizers, Nanjing 210095, China | | | YANG Tianjie | College of Resources and Environmental Sciences, Nanjing Agricultural University
Jiangsu Provincial Key Lab of Solid Organic Waste Utilization
Jiangsu Collaborative Innovation Center of Solid Organic Wastes
Educational Ministry Engineering Center of Resource-saving Fertilizers
National Engineering Research Center of Organic-based Fertilizers, Nanjing 210095, China | | | JIANG Gaofei | College of Resources and Environmental Sciences, Nanjing Agricultural University
Jiangsu Provincial Key Lab of Solid Organic Waste Utilization
Jiangsu Collaborative Innovation Center of Solid Organic Wastes
Educational Ministry Engineering Center of Resource-saving Fertilizers
National Engineering Research Center of Organic-based Fertilizers, Nanjing 210095, China | | | WEI Zhong | College of Resources and Environmental Sciences, Nanjing Agricultural University
Jiangsu Provincial Key Lab of Solid Organic Waste Utilization
Jiangsu Collaborative Innovation Center of Solid Organic Wastes
Educational Ministry Engineering Center of Resource-saving Fertilizers
National Engineering Research Center of Organic-based Fertilizers, Nanjing 210095, China | | | XU Yangchun | College of Resources and Environmental Sciences, Nanjing Agricultural University
Jiangsu Provincial Key Lab of Solid Organic Waste Utilization
Jiangsu Collaborative Innovation Center of Solid Organic Wastes
Educational Ministry Engineering Center of Resource-saving Fertilizers
National Engineering Research Center of Organic-based Fertilizers, Nanjing 210095, China | ycxu@njau.edu.cn | | SHEN Qirong | College of Resources and Environmental Sciences, Nanjing Agricultural University
Jiangsu Provincial Key Lab of Solid Organic Waste Utilization
Jiangsu Collaborative Innovation Center of Solid Organic Wastes
Educational Ministry Engineering Center of Resource-saving Fertilizers
National Engineering Research Center of Organic-based Fertilizers, Nanjing 210095, China | |
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| Abstract: |
| This study aimed to screen and construct a bacterial-fungal consortium that has highly efficient at degrading rice straw. Strawdegrading bacteria and fungi were isolated from the degraded rice straw. Two species each of bacteria and fungi were selected to create the microbial consortia based on the degradation rate. Full combinations of these four straw-degrading microbes, once isolated, were used to construct 15 consortia of one to four species. The degradation rate of the rice straw and the activity of FPase, carboxymethyl cellulase (CMCase), and xylanase were examined to evaluate the degradation ability of the microbial consortia. Linear and multiple regression analyses were then performed to investigate the relationships between microbial consortia and their cellulase activity. A total eight strawdegrading microbes, including four bacteria and four fungi, were isolated from the surface of the rice straw. Among them, Providencia rettgeri BB18, Lysinibacillus sphaericus JB7, Aspergillus fumigatus ZL, and Penicillium oxalicum ZA demonstrated high degradation rate and FPase activity. The results showed that the degradation rate and activities of FPase, CMCase, and xylanase increased with species richness of the microbial consortia. The bacterial-fungal consortium BF1 exhibited the best straw degradation ability; for this group, compared to single microbes, degradation rate increased by 1.6 times and total cellulase activity increased by 1~3 times. Linear regression analysis revealed that the degradation rate of microbial consortia was positively correlated with cellulase activity. CMCase and xylanase were identified as the major contributors to straw degradation by microbial consortia. This study revealed a clear effect of microbial diversity on the ability of bacterial-fungal consortia to degrade rice straw and the cellulase activity of the former. CMCase and xylanase drove the ability of the consortia to degrade the rice straw. |
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