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| Effects of the combination of microplastics and lead pollution on growth and oxidative responses of rice seedlings' roots |
| Received:May 05, 2021 |
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| KeyWord:rice;polystyrene microplastics;lead;oxidative response;synergistic effect |
| Author Name | Affiliation | E-mail | | LIU Ling | School of Biological Engineering, Huainan Normal University, Huainan 232038, China
Key Laboratory of Bioresource and Environmental Biotechnology of Anhui Higher Education Institutes, Huainan 232038, China | | | HONG Tingting | School of Biological Engineering, Huainan Normal University, Huainan 232038, China | | | HU Qiannan | School of Biological Engineering, Huainan Normal University, Huainan 232038, China | | | XIE Ruili | School of Biological Engineering, Huainan Normal University, Huainan 232038, China | | | ZHOU Ying | School of Biological Engineering, Huainan Normal University, Huainan 232038, China | | | WANG Ling | School of Biological Engineering, Huainan Normal University, Huainan 232038, China | | | WANG Chengrun | School of Biological Engineering, Huainan Normal University, Huainan 232038, China | chengrunwang@163.com |
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| Abstract: |
| In order to investigate the effect of polystyrene microplastics(PS-MPs), lead(Pb), and their combination on the growth and oxidative responses of rice seedling roots, changes in root lengths, growth, oxidative damage, antioxidant enzyme activities, and Pb concentrations were examined in the roots of rice seedlings hydroponically cultured in 10~40 mg·L-1 PS-MPs with 20 μmol·L-1 Pb, and their combined solutions. The results showed that, in comparison to the control, the fresh weight, dry weight, and root length were decreased, whereas the products of malondialdehyde(MDA) and superoxide free radicals(O2-·) and root tip necrosis were significantly increased under cultivation with PS-MPs. Meanwhile, the activities of superoxide dismutase(SOD) and ascorbate peroxidase(APX) were differentially elevated in the roots following PS-MP treatment. Compared with Pb treatment alone, treatment with 10~20 mg·L-1 PS-MPs+ Pb resulted in a reduction of Pb accumulation, an increase in root length and fresh weight, and decrease in MDA and O2-·products and alleviation of oxidative damage in the roots. However, 40 mg·L-1 PS-MPs+Pb improved the Pb concentration, diminished the root length and fresh weight, and elevated MDA and O2-· products and aggravated necrosis in the roots. Moreover, higher concentrations of PS-MPs, in combination with Pb, also led to a decline in SOD and catalase activities but enhanced the guaiacol peroxidase activity in the roots. These results indicate that the tested PS-MPs cause oxidative stress and inhibite the growth of the rice seedling roots. PS-MPs at lower concentrations alleviate the Pb-induced oxidative stress, whereas at higher concentrations, it might produce a synergistic effect with Pb, thus aggravating the oxidative damage caused by Pb exposure in the roots of rice seedlings. |
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