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| Microbial community response to volatile fatty acid inhibition in dry anaerobic fermentation |
| Received:May 06, 2021 |
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| KeyWord:dry anaerobic fermentation;volatile fatty acid inhibition;pig manure;straw;microbial community |
| Author Name | Affiliation | E-mail | | WANG Siqi | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | ZHANG Keqiang | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | KONG Dewang | Hangzhou Energy Environmental Engineering Co, Ltd, Hangzhou 310020, China | | | GAO Wenxuan | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | WEI Hong | Huabiao(Tianjin)Technology Co, Ltd, Tianjin 300392, China | | | LIANG Junfeng | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | LI Jiajia | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | DU Lianzhu | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | dulianzhu99@163.com |
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| Abstract: |
| This study investigated the effect of volatile fatty acid(VFA)inhibition on microorganisms in a dry anaerobic fermentation system. Pig manure(group P)and a mixture of pig manure and straw(volatile solid ratio 1∶1, group M)were used as raw materials for this experiment. The microbial community response to VFA inhibition was analyzed by combining gas production performance and physical and chemical indicators. The results showed that both fermentation systems were significantly inhibited by VFA, where the inhibitory effect of group M was more obvious; this was reflected by its specific methane yield, which was < 0.99 mL·g-1. The results from high-throughput sequencing showed that under VFA inhibition, Firmicutes was the main bacterial phylum in the two fermentation systems, and the most abundant genus was Clostridium sensu stricto. Euryarchaeota dominated the archaeal community, whereas the hydrogenotrophic methanogen, Methanosphaerula, and the aceticlastic methanogen, Methanothrix, were the dominant genera. Hydrogenotrophic methanogenesis was the principle pathway for methane formation in the co-digestion system. The main acid inhibitors produced in the two systems differed; in group P it was propionic acid, whereas it was acetic acid for group M. In this experiment, the mixture of pig manure and straw had a stronger acid inhibitory effect than pig manure alone. Additionally, the synergistic effect of bacteria and archaea was hindered, seriously affecting the gas production performance of the system. The occurrence of acid suppression greatly influenced the succession of bacterial flora. |
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