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| Isolation, identification, and degradation capabilities of the herbaceous plant rhizosphere on di -(2-ethylhexyl) phthalate |
| Received:October 27, 2021 |
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| KeyWord:di-(2-ethylhexyl) phthalate(DEHP);degrading strain isolation;herbaceous plant;rhizosphere soil;degradation characteristic;degradation condition |
| Author Name | Affiliation | E-mail | | LIU Shujuan | College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China
College of Chemistry, Biology, and Environment, Yuxi Normal University, Yuxi 653100, China | | | ZHANG Cuiping | College of Chemistry, Biology, and Environment, Yuxi Normal University, Yuxi 653100, China | | | LI Shuying | College of Chemistry, Biology, and Environment, Yuxi Normal University, Yuxi 653100, China | | | LI Xueru | College of Teacher Education, Yuxi Normal University, Yuxi 653100, China | | | LI Yuan | College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China | 374466325@qq.com | | ZHOU Yuanqing | College of Chemistry, Biology, and Environment, Yuxi Normal University, Yuxi 653100, China | yqzhou@yxnu.edu.cn |
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| Abstract: |
| The potential of the herbaceous plant rhizosphere to induce microbial remediation of di-(2-ethylhexyl) phthalate(DEHP) in wetlands contaminated by phthalate esters(PAEs) was researched. DEHP was selected as a target pollutant and B6, a strain capable of degrading it efficiently, was isolated by culture methods from the rhizosphere soil of different herbaceous plants in the contaminated wetlands.Based on its morphology, physiology, biochemical characteristics and an 16S rDNA sequencing analysis, B6 was identified as Bacillus simplex. Simultaneously, the DEHP degradation ability of this strain under different initial DEHP concentrations, pH, inoculum amount, and temperature was studied.The optimal degradation conditions for strain B6 were found to be as follows:initial concentration of 100 mg·L-1, pH 7.0, inoculum amount 4%, and temperature 30℃.Under these conditions, the DEHP degradation rate was 97.91% after 7 days in an inorganic salt medium.Our results indicated that B6 has a significant remediation effect and certain potential for application towards remediating DEHP pollution. This study could provide a theoretical basis for the removal of PAE pollution using constructed herbaceous vegetation wetlands. |
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