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| Effects of continuous cropping and rotation on rhizosphere bacterial community structure of millet |
| Received:June 07, 2021 |
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| KeyWord:millet;continuous cropping;rhizosphere soil;high-throughput sequencing;physicochemical property;enzyme activity |
| Author Name | Affiliation | E-mail | | HAO Xiaofen | Millet Research Institute of Shanxi Agricultural University, Changzhi 046011, China | | | WANG Genquan | Millet Research Institute of Shanxi Agricultural University, Changzhi 046011, China | gqwang1111@163.com | | GUO Erhu | Millet Research Institute of Shanxi Agricultural University, Changzhi 046011, China | guoerhu2003@163.com | | YANG Huiqing | Millet Research Institute of Shanxi Agricultural University, Changzhi 046011, China | | | ZHANG Aiying | Millet Research Institute of Shanxi Agricultural University, Changzhi 046011, China | | | CHENG Qiaolin | Millet Research Institute of Shanxi Agricultural University, Changzhi 046011, China | | | WANG Xiaoyu | Millet Research Institute of Shanxi Agricultural University, Changzhi 046011, China | | | QIN Yuzhong | Millet Research Institute of Shanxi Agricultural University, Changzhi 046011, China | |
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| Abstract: |
| This study explored the effects of continuous millet cropping and rotation on soil bacterial community composition. Highthroughput sequencing technology combining soil physicochemical properties and enzymatic activities were used to analyze the diversity of soil bacterial community composition in abandoned, maize-millet rotation, and continuous cropping land with millet for 2, 3, and 5 years. The soil of millet continuous cropping and crop rotation was alkaline, with its pH ranging from 8.26 to 8.49. The alkali solution nitrogen, organic matter, and urease activity were highest in the rotation land, which began to reduce in 2-year continuous cropping soil, achieved the minimum in 3-year continuous cropping land, and rose again after continuous cropping for 5 years. The rapidly available potassium, polyphenol oxidase, catalase, and invertase values in the continuous cropping lands were higher than in the rotation land. The community composition of soil bacteria was fixed at the phylum level between continuous cropping and rotation of millet, but the abundance of different species differed significantly. Results showed that Actinobacteria, Proteobacteria, and Acidobacteria were the dominant bacteria in millet rhizosphere soil. The relative abundance of Proteobacteria, Acidobacteria, Bacteroides, Firmicutes, and Nitrocellulobacteria in rotation soil was higher than in the continuous cropping soil, whereas hexane and Chlorocurved bacteria in the rotation soil were lower. Alpha diversity analysis showed no significant difference among the groups, whereas Beta diversity analysis showed a significant difference between continuous cropping, maize-millet rotation, and abandoned land. Redundancy analysis showed that Sphinomonas was positively correlated with pH, rapidly available potassium, organic matter, and alkaline hydrolysis nitrogen, whereas Nocarnoid was positively correlated with pH, rapidly available phosphorus, organic matter, alkaline hydrolysis nitrogen, urease, and catalase. There were differences in the dominant bacteria of rhizosphere soil among the rotation, continuous cropping, and abandoned land groups. The linear discriminant analysis effect size determined specific soil markers in the rhizosphere soil, that the dominant bacteria in the rotation soil were Sphingomonas and Nocardioides, and that the dominant bacteria in the continuous cropping land for 3 years was Solirubrobacter. In conclusion, with the increase of continuous millet cropping years, the soil nutrients first decrease and then increase. The ASV abundance of bacteria decrease compared with rotation, and the bacterial community distribution is significantly different. |
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