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| Screening, identification, and degradation performance of a dimethyl disulfide degrading strain |
| Received:August 04, 2021 |
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| KeyWord:dimethyl disulfide(DMDS);degrading microbe;16S rDNA;isolation;identification;degrading characters |
| Author Name | Affiliation | E-mail | | DING Jun | Department of Agricultural Resources and Environment, College of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing 210044, China | | | WU Hongsheng | Department of Agricultural Resources and Environment, College of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing 210044, China | wuhsluck@163.com | | SUN Qian | Department of Agricultural Resources and Environment, College of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing 210044, China | | | WANG Na | Nanjing Institute of Environmental Sciences, Ministry of National Ecology and Environment of China, Nanjing 210012, China | wangna@nies.org | | CHENG Cheng | Department of Agricultural Resources and Environment, College of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing 210044, China | | | SHI Taoran | Department of Agricultural Resources and Environment, College of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing 210044, China | | | Muhammad Faheem | Department of Agricultural Resources and Environment, College of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing 210044, China | | | NI Ni | Nanjing Institute of Environmental Sciences, Ministry of National Ecology and Environment of China, Nanjing 210012, China | | | TIAN Wei | Nanjing Institute of Environmental Sciences, Ministry of National Ecology and Environment of China, Nanjing 210012, China | | | WU Yuncheng | Nanjing Institute of Environmental Sciences, Ministry of National Ecology and Environment of China, Nanjing 210012, China | | | SHAN Zhengjun | Nanjing Institute of Environmental Sciences, Ministry of National Ecology and Environment of China, Nanjing 210012, China | |
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| Abstract: |
| To obtain dimethyl disulfide(DMDS) strain resources efficiently and reduce the adverse effects of its residues in the environment, this study isolated and purified a bacterium capable of degrading DMDS in the soil near an abandoned agricultural pharmaceutical factory in Suzhou. The molecular biology, physiological, and biochemical identification of bacteria were performed to study their degradation performance under different initial concentrations, rotational speeds, pH, temperature, and added carbon and nitrogen sources. The bacterium was named SZT-1, and the bacterial gene sequence had a 98% homology with Bacillus sp. based on phenotypic analysis and homology comparison using BLAST and 16S rDNA identification. The SZT-1 strain grew on a mineral medium with a sole carbon source of DMDS and reached a stable growth stage after 56 h. The strain effectively degraded DMDS based on flask-shaking culture and order 1 dynamic analysis where the halftime of DMDS degradation was decreased from 346.5 h to 86.6 h. The maximal DMDS degrading rate(approximately 50%) was measured using environmental condition single factor analysis at an initial concentration of 250 mg·L-1 DMDS, rotation speed of 130 r·min-1, pH of 5, temperature of 30℃, exogenously addition of starch as the carbon source and peptone. To date, little is known regarding DMDS biodegradation, with the findings from this study of SZT-1 degrading DMDS the first reported. This study provides a reference for the biodegradation of VOC odor pollutants that could be used to control and decrease the harm by DMDS in the soil and groundwater environment in the future. |
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