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| The mitigation effect of brassinolide on phytotoxicity of tribenuron–methyl·carfentrazone-ethyl·MCPA-Na on broomcorn millet |
| Received:July 22, 2021 |
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| KeyWord:broomcorn millet;brassinolide;WP mixture of tribenuron –methyl·carfentrazone-ethyl ·MCPA-Na;phytotoxicity mitigation;active oxygen metabolism |
| Author Name | Affiliation | E-mail | | LIN Ruichang | College of Agronomy, Northwest A&F University/State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China | | | GAO Yumeng | College of Agronomy, Northwest A&F University/State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China | | | WANG Kexin | College of Agronomy, Northwest A&F University/State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China | | | LI Xin | College of Agronomy, Northwest A&F University/State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China | | | YANG Simin | College of Agronomy, Northwest A&F University/State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China | | | GAO Xiaoli | College of Agronomy, Northwest A&F University/State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China | gao2123@nwsuaf.edu.cn |
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| Abstract: |
| This study evaluated the mitigation effect of brassinolide（BR）on the phytotoxicity of tribenuron–methyl·carfentrazone-ethyl· MCPA-Na to provide a theoretical basis for the secure application of herbicides and the mitigation of herbicide phytotoxicity in broomcorn millet fields. Yumi 2 was planted in an experimental field located in Yulin, Shaanxi, from June to October 2020. Plants at the three-leaf to four-leaf stage were treated with 0.6 kg·hm-2 WP mixture of tribenuron–methyl·carfentrazone-ethyl·MCPA-Na. After 3 d, 0.06 mg·L-1 of BR, 0.08 mg·L-1 of BR, and 0.10 mg·L-1 of BR were foliarly applied, respectively. The phytotoxicity and fresh weight control effects of weeds were investigated for each treatment group. We measured the chlorophyll content and level of antioxidant enzymes in the functional leaves of broomcorn millet as well as the overall yield. Phytotoxicity showed a negative correlation with BR concentration. Compared with CK herbicide application, phytotoxicity indexes were reduced by up to 11.34, 13.26, and 12.75 percent points, respectively, depending on BR concentration. This was accompanied by a slightly reduced weed control effect. However, there was no significant difference from single application of herbicide. The WP mixture of tribenuron –methyl·carfentrazone-ethyl·MCPA-Na decreased plant height, stem thickness, ear length, main ear weight, yield, the activity levels of peroxidase（POD）, superoxide dismutase（SOD）, catalase（CAT）, glutathione Stransferase（GST）, as well as chlorophyll and glutathione（GSH）content, but significantly increased malondialdehyde（MDA）content. The BR treatments increased the yield, activity levels of reactive oxygen metabolizing enzymes and GST, content levels of chlorophyll and GSH, and reduced the level of MDA content. The results indicate that treating broomcorn millet with 0.06~0.10 mg·L-1 BR following application of 0.6 kg·hm-2 WP mixture of tribenuron–methyl·carfentrazone-ethyl·MCPA-Na can maintain the balance of cell redox by increasing the activity of antioxidant enzymes in the leaves and improving the activity of GST to promote the formation of an avirulence conjugate related to reduced glutathione, alleviating damage while promoting the growth and yield of broomcorn millet. The BR concentration of 0.08 mg·L-1 shows the best effect, alleviating the phytotoxicity, while promoting the yield of broomcorn millet. |
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