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| Isolation and identification of efficient organophosphate-dissolving bacteria from rhizosphere soil of P-efficient transgenic rice OsPT4 |
| Received:August 20, 2021 |
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| KeyWord:P-efficient transgenic rice;organophosphate-solubilizing bacteria;16S rDNA |
| Author Name | Affiliation | E-mail | | ZHANG Le | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
Key Laboratory of Environmental Pollution Prevention and Control in Production Area, Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Agricultural Environment and Agricultural Product Safety, Tianjin 300191, China | | | ZHANG Siyu | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
Key Laboratory of Environmental Pollution Prevention and Control in Production Area, Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Agricultural Environment and Agricultural Product Safety, Tianjin 300191, China | | | WANG Yalu | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
Key Laboratory of Environmental Pollution Prevention and Control in Production Area, Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Agricultural Environment and Agricultural Product Safety, Tianjin 300191, China | | | LI Gang | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
Key Laboratory of Environmental Pollution Prevention and Control in Production Area, Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Agricultural Environment and Agricultural Product Safety, Tianjin 300191, China | | | YANG Dianlin | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
Key Laboratory of Environmental Pollution Prevention and Control in Production Area, Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Agricultural Environment and Agricultural Product Safety, Tianjin 300191, China | | | ZHAO Jianning | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
1. Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | zhaojn2008@163.com |
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| Abstract: |
| P-efficient transgenic rice OsPT4 shows a relatively high consumption rate of available phosphorus. To increase P-utilization, organophosphate-dissolving bacteria were isolated from the rhizosphere soil of P-efficient transgenic rice OsPT4 by Pikovskaya organophosphorus solid medium and classified by 16S rDNA gene sequencing. Fifteen organophosphate-dissolving strains were isolated, of which six produced clear transparent circles on the organophosphorus medium and were used for purification and rescreening. Their solubility index ranged from 1.50~6.33, and strain Y7 showed the highest solubility. Phosphate-dissolving capacity, after being cultured for 24 h, ranged from 10.60~87.43 mg·L-1, and strain Y7 showed the highest capacity. After culturing for 24 h, strain Y7 showed the highest pH reduction, and the second highest alkaline phosphatase secretion（after strain Y11）. Five of the strains（excluding Y7）were common groups that could be screened from traditional field crop soil and they showed no difference in P-solubilizing ability or mechanism. Strain Y7 was identified as Pantoea sp., a common core group of endophytic bacteria in rice seeds. Its phosphorolytic mechanism was the interaction of pH reduction and alkaline phosphatase secretion, which could be used as a resource for efficient phosphorolytic bacteria in future research. |
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