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| Effects of cadmium stress on cadmium subcellular distribution and non-protein thiol of Agaricus brasiliensis |
| Received:October 12, 2021 Revised:December 15, 2021 |
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| KeyWord:Agaricus brasiliensis;cadmium;subcellular distribution;non-protein thiols compound |
| Author Name | Affiliation | E-mail | | ZHOU Yu | College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
National Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China | | | ZHANG Conghui | College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
National Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China | | | WENG Boqi | Agricultural Ecology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China | | | CHEN Hua | Agricultural Ecology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China | | | LIU Penghu | National Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China | phliu1982@163.com |
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| Abstract: |
| In this study, Agaricus brasiliensis strains J1(high Cd-accumulating strain) and J77(low Cd-accumulating strain) under different concentrations of Cd stress were used as research materials. Using differential centrifugation and an enzyme-linked immunosorbent assay, we investigated the mycelial growth, Cd subcellular distribution, change in the content of small-molecule nonprotein thiol(NPT)compounds, and activity of small-molecule NPT compound-metabolizing enzymes that were related to intracellular Cd chelation, which revealed the response mechanisms of different Cd accumulating strains to Cd stress at the cellular level and enzymatic activity. The results showed that as the concentration of external Cd increased, the growth of the hyphae of the two strains was inhibited, and many aerial hyphae appeared. There were more aerial hyphae of J77 than that of J1. The distribution of Cd in the hyphae of J1 and J77 followed the order of cell wall > cell sap > organelles, which indicated that the cell wall could hold Cd2+. The activities of glutathione Stransferase and glutathione peroxidase(GSH-Px)in strain J77 were higher than those in J1. The changes in the phytochelatin(PC)and NPT contents in J77 and J1 were consistent, and showed "stimulation at low Cd concentrations and inhibition at high Cd concentrations". Correlation analysis showed that Cd stress was positively correlated with the GSH-Px content, and the NPT content was significantly positively correlated with the PC content. The Cd detoxification mechanism of A. brasiliensis mycelium included cell wall deposition, heavy metal partitioning, and synthesis of NPT substances. Different strains exhibit different response patterns to Cd stress. |
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