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| Stereoselective effects of the chiral triazole fungicide epoxiconazole on soil microbes |
| Received:December 01, 2021 Revised:January 28, 2022 |
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| KeyWord:non-targeted metabolomics;high-throughput sequencing;stereoselectivity;bacteria;fungi;soil metabolome;phylogenetic tree;PICRUSt;fungicide |
| Author Name | Affiliation | E-mail | | XUE Pengfei | Key Laboratory for Environmental Factors Control of Agro-product Quality Safety, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | LIU Xiaowei | Key Laboratory for Environmental Factors Control of Agro-product Quality Safety, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | ZHAO Liuqing | Key Laboratory for Environmental Factors Control of Agro-product Quality Safety, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | HE Zeying | Key Laboratory for Environmental Factors Control of Agro-product Quality Safety, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | hezeying222308@163.com |
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| Abstract: |
| This study aimed to explore the response mechanism of the soil metabolome and microbial communities to epoxiconazole racemates and their enantiomers based on the stereoselective response of soil microorganisms to the chiral triazole fungicide epoxiconazole, using a combination of non-targeted metabolomics and high-throughput sequencing. No significant degradation of epoxiconazole and its enantiomers in the soil was observed after 4 weeks of treatment. This residue could cause significant changes in the soil metabolome and soil microbial community composition. The soil metabolome, bacterial and fungal community composition, and metabolic pathways predicted by the PICRUSt gene showed stereoselective responses driven by 2R, 3S -(+)- epoxiconazole. The MetaCyc pathways of the significant responses of 10 and 22 strips were observed in bacteria and fungi, respectively, as shown by the results of PICRUSt gene prediction. The interference in soil microorganisms by (±) -epoxiconazole and (+) -enantiomers was more significant than by (-) -enantiomers. The bacterial and fungal communities, metabolism profile, and prediction pathways of gene function in the soil environment showed varying degrees of stereoselective response to cis-epoxiconazole. Given the importance of the soil environment in agricultural production, characterization of the soil microbiome and metabolome can provide new insights into the ecological risks posed by exposure to the chiral triazole pesticide cis-epoxiconazole and its enantiomers. |
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