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| Effects of tea polyphenols and calcium ions on absorption and subcellular distribution of lead in tea leaves |
| Received:December 21, 2021 |
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| KeyWord:lead;tea polyphenols;calcium ion;tea leaves;subcellular distribution |
| Author Name | Affiliation | | GE Gaofei | Biotechnology Center of Anhui Agricultural University, Hefei 230036, China | | SHEN Xusong | School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | CHEN Nuo | School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | WANG qin | Biotechnology Center of Anhui Agricultural University, Hefei 230036, China | | XU Yang | Biotechnology Center of Anhui Agricultural University, Hefei 230036, China |
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| Abstract: |
| Hydroponic experiments combined with differential centrifugation and electron microscopy were used to study the characteristics and subcellular distribution of lead absorption in Shuchazao tea leaves under lead stress. Differences in the absorption and subcellular distribution of lead in tea leaves were compared after tea polyphenol and calcium ion addition. Lead stress significantly increased the lead content and decreased the calcium content in tea leaves. After the addition of tea polyphenols, the lead content in tea leaves decreased significantly and the calcium content increased significantly;the reduction rate of lead and increase rate of calcium were 30.89% and 11.10%, respectively. The addition of calcium ions had no significant effect on the lead content in tea leaves, but significantly increased the calcium content under lead stress, with an increase rate of 34.57%. The absorption of lead by tea plants increased the folds on the surface of leaves and around stomata, and lead was deposited in vacuoles, causing damage to organelles. The addition of tea polyphenols and calcium ions reduced cell damage due to lead stress. The distribution order of lead in the subcellular components of tea leaves was cell wall, cytoplasm and vacuole, plastid and chloroplast, and mitochondria. Tea polyphenols primarily alleviate the lead toxicity of tea leaves by reducing lead absorption, and calcium ions alleviate the toxic effect of lead on tea leaf cells by altering the lead content in different components of tea leaf cells(increasing the interception of lead by the cell wall and reducing the lead content in cells). |
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