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| Metabolic damage mechanism of different concentrations of PFOS on Arabidopsis thaliana leaves |
| Received:February 28, 2022 |
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| KeyWord:metabolomics;Arabidopsis thaliana;perfluorooctane sulfonates(PFOS);ecotoxicity |
| Author Name | Affiliation | E-mail | | ZHANG Yue | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | LIU Xiaowei | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | ZHANG Yanwei | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | zhangyanwei14@163.com | | ZHANG Jingran | Shanghai SCIEX Analytical Instrument Trading Co., Ltd., Shanghai 200335, China | | | LIU Bingjie | Shanghai SCIEX Analytical Instrument Trading Co., Ltd., Shanghai 200335, China | |
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| Abstract: |
| Information regarding the toxic effects of high concentration of perfluorooctane sulfonates(PFOS)on plants cannot be used as the standard for determining the toxic effects of low concentration of PFOS on the environment. Moreover, the various toxic effects of different levels of PFOS are still not clear. To better understand the toxic effects of different concentrations of PFOS on plants, a highthroughput non-targeted metabolomics technique was used to study the metabolic effects of different concentrations of PFOS on Arabidopsis thaliana. Several differential metabolites were screened through sterile pot experiments using exposure and control groups. Data were analyzed statistically using methods, such as PCA, OPLS-DA, VIP, and t-test. PCA analysis showed significant separation between the high and low exposure groups. 11~24 biomarkers were found in the low-exposure groups(0.1 mg · L-1 and 1 mg · L-1), whereas 23~29 biomarkers were found in the high-exposure groups(5, 10 mg·L-1, and 20 mg·L-1). The disturbed metabolic pathways in the low exposure group were phenylpropanoid biosynthesis in oxidative stress and sugar metabolism in energy metabolism, whereas the disturbed metabolic pathways in the high exposure group were amino acid metabolism; unsaturated fatty acid, aminoacyl tRNA, phenylpropanoid, cutin, suberine, wax, fatty acid, and canola glycerol biosynthesis; and α-linolenic acid and sulfur metabolism. This study revealed that high and low levels of PFOS caused different metabolic toxicity in Arabidopsis thaliana leaves, and Arabidopsis thaliana initiated different metabolic defense mechanisms against different concentrations of PFOS. The results provide theoretical basis for studying the toxic effects of different levels of perfluorinated compounds on the environment. |
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