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| Antioxidant enzyme-related genes of Pogonatherum crinitum leaves in response to lead stress based on RNA-Seq |
| Received:March 05, 2022 Revised:April 15, 2022 |
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| KeyWord:hyperaccumulator;Pogonatherum crinitum;Pb stress;antioxidase;qRT-PCR |
| Author Name | Affiliation | E-mail | | ZHU Chenlu | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China | | | WU Xinyi | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China | | | YU Junbao | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China | | | HUANG Siqi | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China | | | CAO Shuyi | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China | | | ZHAI Lin | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China | | | HAN Xuejie | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China | | | HOU Xiaolong | College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Key Laboratory of State Administration of Forestry and Grassland on Soil and Water Conservation of Red Soil Region in Southern China, Fuzhou 350002, China
Cross-Strait Collaborative Innovation Center of Soil and Water Conservation, Fuzhou 350002, China | xl.hou@fafu.edu.cn |
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| Abstract: |
| To further reveal the related genes of plant antioxidant enzymes and their mechanisms of response to heavy metal stress,Pogonatherum crinitum was used as the research object, and a hydroponic simulated stress experiment with lead concentrations of 0, 300, 500, 1 000 mg·L-1, and 2 000 mg·L-1 was carried out. In addition, the antioxidant enzyme activity in the leaves of P. crinitum at different concentrations was determined, and the sequencing of the RNA-Seq of P. crinitum leaves by Pb treatments was performed. The unigenes obtained by transcriptome sequencing were enriched and annotated with GO and KEGG, and the DEGs related to leaf antioxidant enzymes were screened out and verified using qRT-PCR. The results revealed that, with the increase in lead stress concentration, the activities of POD, SOD, and CAT in the leaves of P. crinitum showed an increasing trend; however, MDA content showed an increasing trend firstly and then decreased. The total number of bases obtained by sequencing was 56.34 Gb. The base quality of each sample reached Q20 and Q30 exceeded 90%, indicating that the database was reliable. The selected DEGs were compared and annotated through GO and KEGG databases, and the result showed that antioxidant-related GO functional terms such as“peroxisome” “oxidoreductase activity”and “oxidoreductase activity, acting on CH-OH group of donors” “reactive oxygen species metabolic process”and“hydrogen peroxide metabolic process”were significantly enriched in the DEGs of P. crinitum. Moreover, the DEGs were significantly enriched in antioxidantrelated KEGG pathways such as“glutathione metabolism” “ascorbate and aldarate metabolism” “plant hormone signal transduction” "flavonoid biosynthesis”and“MAPK signaling pathway-plant” . The expression of antioxidant enzyme-related genes PcSOD, PcPOD, and PcCAT in the leaves of P. crinitum were upregulated under Pb stress. The qRT-PCR results were consistent with the transcriptome sequencing results, and the relative expression levels of these genes were consistent with the changing trend of the antioxidant enzyme activities under Pb stress. This study shows that P. crinitum adapts to heavy metal Pb stress by increasing the activity of antioxidant enzymes, and the antioxidant enzyme-related genes PcSOD, PcPOD, and PcCAT are involved in the regulation of P. crinitum in response to heavy metal Pb stress. |
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