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| Screening of sulfur-oxidizing bacteria and biological oxidation characteristics |
| Received:May 10, 2022 Revised:August 17, 2022 |
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| KeyWord:livestock and poultry production;hydrogen sulfide;deodorization;sulfur-oxidizing bacteria;Halomonas |
| Author Name | Affiliation | E-mail | | YU Shuhao | College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China
Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | ZHAI Zhongwei | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | SHEN Fengju | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | LIANG Junfeng | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | liangjunfeng@caas.cn | | ZHANG Keqiang | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | kqzhang@126.com | | LI Mingtang | College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China | | | WANG Rui | Agricultural Environmental Protection Station of Xian'an District, Xianning City, Xianning 437000, China | |
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| Abstract: |
| To reduce and control malodorous hydrogen sulfide gas emissions from livestock and poultry farms, we screened a highefficiency sulfur-oxidizing bacterium according to two indicators： S2- oxidation performance and hydrogen-sulfide-emission reduction in manure. In addition, we studied the biological oxidation characteristics and influencing factors of the strain. The results showed that we had isolated a highly efficient heterotrophic sulfur-oxidizing strain from the sewage storage tank of a dairy farm, which had a strong S 2- oxidation capacity and reduced the hydrogen sulfide in the pasture sewage by 47.1%. We identified this strain as Halomonas, which we named Halomonas sp. AEB2. Based on the results of single-factor tests, we optimized the environmental S2- oxidation parameters of strain AEB2 using the Box–Behnken response surface methodology. The results showed that the factors affecting the sulfide removal rate were temperature > pH > rotational speed, and the optimal environmental parameters were a temperature of 32.5 ℃, speed of 210 r·min-1, and initial pH of 8.2. The sulfide removal rate of strain AEB2 was more than 99% when the S2- load was 100, 200, 500 mg·L-1, and 1 000 mg· L-1; the fitting results of the OPED and PFOPD models showed that the oxidation rate constants of strain AEB2 under the various loading conditions were 1.36, 0.83, 0.38 h-1, and 0.19 h-1, respectively. AEB2 has a high S2- oxidizing ability and can be used as an engineered bacterial strain for hydrogen-sulfide-odor removal. |
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