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| Exogenous Ca2+ application regulates nitrogen uptake in roots of different resistant rice varieties under simulated acid rain stress |
| Received:March 15, 2022 |
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| KeyWord:exogenous Ca2+;simulated acid rain;rice;differences in acid tolerance;nitrogen uptake;plasma membrane H+-ATPase phosphorylation level |
| Author Name | Affiliation | E-mail | | ZHANG Yuanqi | Jiangsu Province Key Laboratory of Anaerobic Biological Technology, School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China | | | LIANG Chanjuan | Jiangsu Province Key Laboratory of Anaerobic Biological Technology, School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China
Jiangsu Water Treatment Technology and Materials Collaborative Innovation Center, Suzhou University of Science and Technology, Suzhou 215009, China | liangchanjuan@jiangnan.edu.cn |
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| Abstract: |
| To alleviate the adverse effect of acid rain on plant growth, the regulation mechanism of Ca2+ on tolerance of plants to acid was explored. In this study, two rice cultivars, Wuyou 308（resistant cultivar）and Nanjing 9108（sensitive cultivar）, were selected as research objects. The effect of exogenous Ca2+ on root growth, nitrogen（NO3- and NH4+）content and uptake rates, ATP content, and plasma membrane H+-ATPase activity and phosphorylation levels in rice seedlings under low-intensity（pH 4.5, SAR1）and high-intensity（pH 3.0, SAR2） simulated acid rain were studied. The results showed that under SAR1 treatment, H+-ATPase phosphorylation levels and activity increased in the two rice cultivars, which promoted the decomposition of ATP and increased energy supply, thereby inducing an increase in NH4+ uptake. However, the accumulation of NH4+ was excessive only in Nanjing 9108, causing ammonium toxicity and inhibiting root growth. Under the same treatment, NO3- and NH4+ levels and growth did not change in Wuyou 308. Under SAR2 treatment, H+-ATPase activity and NO3- and NH4+ uptake rates and levels as well as root growth decreased in the two rice cultivars. Among them, the degree of decrease in Nanjing 9108 was greater than that in Wuyou 308. Under Ca2++SAR1 treatment, H+-ATPase phosphorylation levels and activity in the root plasma membrane, uptake and accumulation of NO3- and NH4+, as well as root growth of the two rice cultivars were not significantly different from those in the control（the treatment which leaves sprayed deionized water）. The H+-ATPase activity, uptake and accumulation of NO3- and NH4+, and root growth of the two rice cultivars under Ca2++SAR2 treatment were lower than those in the control, but significantly higher than those under SAR2 treatment alone. The results showed that exogenous Ca2+ effectively guaranteed the phosphorylation levels of H+-ATPase and promoted H+-ATPase activity, and then relieved the inhibition of NO3- and NH4+ uptake and maintained root growth under simulated acid rain（pH 4.5 and 3.0）. Among treatments, at the same simulated acid rain intensity, regulation of exogenous Ca2+ in Wuyou 308 was better than that in Nanjing 9108, indicating that the effect of exogenous Ca2+ on nitrogen uptake under simulated acid rain was not only limited by acid rain intensity, but also exhibited differences in cultivars. In this experiment, exogenous Ca2+ has a regulatory effect on the nitrogen uptake process of different resistant rice under acid rain stress of different intensities, and the rational application of exogenous Ca2+ can help regulate the nutrient uptake of crops in areas with acid rain to alleviate the damage caused by acid rain to agricultural production. |
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