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| Study on the accumulation of tetracycline resistance genes in soil with different utilization patterns in China |
| Received:March 11, 2022 |
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| KeyWord:tetracyclines resistance gene;facility vegetable field;open vegetable field;open grain field |
| Author Name | Affiliation | E-mail | | GONG Xiaoya | College of Horticulture and Landscape, Northeast Agricultural University, Harbin 150030, China | | | SONG Jianyu | College of Horticulture and Landscape, Northeast Agricultural University, Harbin 150030, China | | | WU Fengzhi | College of Horticulture and Landscape, Northeast Agricultural University, Harbin 150030, China | fzwu2006@aliyun.com |
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| Abstract: |
| Variations in the absolute abundance of antibiotic resistance genes in different regions of China under different land-use modes and planting years were explored. The purpose of this study is to reveal the accumulation of resistant genes in cropland. Soil from 14 provinces, including Heilongjiang（HLJ）, Jilin（JL）, Liaoning（LN）, Inner Mongolia（NMG）, Xinjiang（XJ）, Gansu（GS）, Yunnan（YN）, Jiangsu（JS）, Hubei（HB）, Guangdong（GD）, Hainan（HN）, Shanxi（SX）, Henan（HEN）, and Shandong（SD）were used in this study. There were nine treatments in total based on different land-use modes（open grain fields, open vegetable fields, and facility vegetable fields）and planting years（1-3, 7-10 years, and more than 15 years）. Tetracycline resistance genes were qualitatively and quantitatively analyzed by polymerase chain reaction（PCR）and quantitative PCR. The absolute abundance of tetracycline resistance genes（tetM, tetO, tetQ, and tetW）in facility soil was significantly higher than that in open grain and vegetable field soil. The absolute abundance in facility vegetable soil was 3.96×104-6.36×108 copies·g-1; in open grain fields, it was 2.16×103-2.18×106 copies·g-1. In open vegetable fields, it was 1.16× 104-2.48 × 107 copies · g-1. The highest absolute abundance of resistance genes appeared in different provinces under different land-use modes and planting years, respectively. However, the greenhouse soil of year 3, 7, and 15 in provinces was significantly higher than that of open grain and vegetable fields for the same years. The facility vegetable soil in years 7 and 15 was significantly higher than in year 3. There was no significant linear relationship between gene accumulation and planting life in open grain and vegetable fields, despite a significantly positive correlation in facility vegetable soil. Tetracycline resistance genes have been ubiquitous in Chinese soil under different land-use modes and planting years. Its abundance was significantly higher in facility vegetable field soil than in open vegetable and open grain field soil. The abundance of facility vegetable fields was significantly positively related to soil planting life. |
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