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| Distribution characteristics and influencing factors of antibiotic resistance genes in sediments from large yellow croaker（Larimichthys crocea）culture areas |
| Received:July 06, 2022 |
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| KeyWord:antibiotic resistance gene;macro-genome sequencing analysis technology;large yellow croaker culture area;sediment;microbial community |
| Author Name | Affiliation | E-mail | | HUANG Wei | Institute of Agricultural Quality Standards and Testing Technology Research, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agro-products Quality & Safety, Fuzhou 350003, China
College of Life Science, Fujian Normal University, Fuzhou 350108, China | | | LIU Yang | Institute of Agricultural Quality Standards and Testing Technology Research, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agro-products Quality & Safety, Fuzhou 350003, China | | | RAO Qiuhua | Institute of Agricultural Quality Standards and Testing Technology Research, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agro-products Quality & Safety, Fuzhou 350003, China | | | LUO Qin | Institute of Agricultural Quality Standards and Testing Technology Research, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agro-products Quality & Safety, Fuzhou 350003, China | | | WANG Weigang | Aquatic Technology Promotion Station of Lianjiang County, Fuzhou 350500, China | | | SONG Yongkang | Institute of Agricultural Quality Standards and Testing Technology Research, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agro-products Quality & Safety, Fuzhou 350003, China | songlibby@sina.com | | LUO Tuyan | Institute of Agricultural Quality Standards and Testing Technology Research, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agro-products Quality & Safety, Fuzhou 350003, China | zxl6588@qq.com |
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| Abstract: |
| To reveal the distribution characteristics and influencing factors of antibiotic resistance genes（ARGs）in the sediments of large yellow croaker culture areas, sediment samples from six sites of large yellow croaker culture areas were collected via field survey, and the distribution characteristics of ARGs in the sediments were analyzed using macro-genome sequencing analysis technology. The environmental factors and microbial community structure in the sediment were analyzed using chemical analysis and high-throughput sequencing techniques, and the correlation between ARGs and environmental factors and microbial community structure was investigated. 781 ARGs subtypes belonging to 38 ARGs types were identified, indicating the wide-spectrum profiles of ARGs in the sediments from large yellow croaker culture areas. Statistical analyses revealed that the types of antibiotic resistance in the dominant ARGs in sediments from different large yellow croaker culture areas were basically the same, mainly tetracyclines, macrolides, and fluoroquinolones, and the main mechanism causing bacterial resistance was the efflux pump. Correlation analysis showed that the top 20 ARGs were not significantly correlated（P>0.05）with environmental factors, except for PNGM-1, which was significantly and positively correlated（P<0.05）with conductivity and TN; however, all the top 20 ARGs were significantly or highly significantly correlated（P<0.05, P<0.01）with the top 20 bacterial genera. Our results indicate that sediments from large yellow croaker culture areas are rich in ARGs, and the microbial community is an important factor that affects the distribution of ARGs in the sediments. |
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