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| Subcellular distribution of arsenic in Sargassum horneri and the role of the cell wall in arsenic biosorption |
| Received:July 18, 2022 |
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| KeyWord:cell subfraction;cell wall chemical modification;sorption kinetics;polysaccharide;FTIR;Sargassum horneri |
| Author Name | Affiliation | E-mail | | ZHANG Peng | Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, Zhejiang Mariculture Research Institute, Wenzhou 325000, China | | | LIU Wei | Marine Science Research Institute of Shandong Province(National Oceanographic Center, Qingdao), Qingdao 266104, China | liuwei_mbi@163.com | | WANG Pengyun | Aquaculture Technology Extension Station of Putian, Putian 351100, China | | | WANG Tiegan | Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, Zhejiang Mariculture Research Institute, Wenzhou 325000, China | | | ZHONG Chenhui | Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361000, China | | | TAO Yueliang | College of Life and Environmental Science, Wenzhou University, Wenzhou 325000, China | |
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| Abstract: |
| In order to reveal the mechanism of arsenic(As) biosorption of the cell wall of Sargassum horneri, we used S. horneri seedlings to determine the variations of As distribution in cell subfraction under different As stress concentrations(50, 100, 150, 200 μmol·L-1, and 250 μmol·L-1). Experiments on the kinetics of the cell wall biosorption, As sorption effect of the polysaccharides extracted from the cell wall, and FTIR analysis were also carried out. The results showed that 50% of As was distributed in the cell wall fraction which was significantly higher than in other cell subfractions under a low level of As stress(50 μmol·L-1). However, with increasing As stress(≥ 150 μmol·L-1), As was dominantly distributed in the soluble fraction instead of the cell wall, in which less deposition was observed(36.4%- 37.3%). Although there was no notable increase in the As content of the cell wall fraction when As stress concentration was above 100 μmol·L-1, the As content of the cell wall fraction tended to increase with increasing As stress overall. Results from the kinetic analysis showed that both the esterification and the methylation of the cell wall decreased the equilibrium capacity of As sorption, which declined by 68.5% and 42.9%, respectively. Moreover, FTIR analysis revealed that functional groups such as hydroxyl, amino, carboxyl, and sulphate on the polysaccharide of the cell wall potentially provided binding sites for As. Sorption experiment in vitro showed that the rate of As sorption gradually increased with the increasing concentration of polysaccharides extracted. Our findings suggest that the cell wall of S. horneri has an important biosorption capacity of As, in which the functional groups on polysaccharides play key roles, and the cell wall can adsorb more As with increasing of polysaccharide content. |
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