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| Effects of precipitation variation and nitrogen deposition on soil bacterial community structure and enzyme activity in desert steppe |
| Received:June 25, 2022 |
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| KeyWord:soil bacterial diversity;soil enzyme activity;nitrogen deposition;precipitation treatment;desert steppe |
| Author Name | Affiliation | E-mail | | TU Nare | Inner Mongolia Agricultural University, Huhhot 010011, China | | | HONG Mei | Inner Mongolia Agricultural University, Huhhot 010011, China
Inner Mongolia Key Laboratory of Soil Quality and Nutrient Resources, Huhhot 010011, China
Key Laboratory of Agricultural Ecological Security and Green Development at Universities of Inner Mongolia Autonomous, Hohhot 010018, China | nmczhm1970@126.com | | YAN Jin | Inner Mongolia Agricultural University, Huhhot 010011, China | | | YE He | Inner Mongolia Agricultural University, Huhhot 010011, China | | | LIANG Zhiwei | Inner Mongolia Agricultural University, Huhhot 010011, China | | | WANG Zhanhai | Hulunbeier Agriculture and Animal Husbandry Technology Promotion Center, Hailar 021008, China | |
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| Abstract: |
| A twelve-treatment experiment was designed in the Stipa breviflora desert steppe, with a main plot[three water treatments:30% increase(W), 30% reduction(R), and natural rainfall(CK)], and a split-plot with four nitrogen gradients[0(N0), 30 kg·hm-2·a-1(N30), 50 kg·hm-2·a-1(N50), and 100 kg·hm-2·a-1(N100)] to investigate the interaction effect of nitrogen deposition and rainfall fluctuations on soil bacterial community structure and enzyme activity. The results revealed that precipitation changes and nitrogen deposition altered the composition of the soil bacterial community, but not the alpha diversity of soil bacteria, and that precipitation changes and different nitrogen treatments had significant effects on soil enzyme activities. Soil catalase activity was the lowest in R-N100(1.63 mg·g-1·d-1) and significantly decreased by 7.4% compared with CK-N0. Soil sucrase activity was the highest in W-N0(2.20 mg·g-1·d-1) and significantly increased by 14.6% compared with CK-N0, and the lowest in R-N100(1.52 mg·g-1·d-1) and significantly decreased by 20.8% compared with CK-N0. Soil urease activity was the highest in W-N0(17.66 mg·g-1·d-1) and significantly increased by 16.7% compared with CKN0, and the lowest in CK-N100(9.27 mg · g-1 · d-1) and significantly decreased by 38.7% compared with CK-N0. Soil catalase was positively correlated with the bacterial richness index, whereas sucrase was positively correlated with both the bacterial diversity index and the bacterial richness index. The structural equation model showed that soil enzyme activity was primarily regulated by the diversity and pH of soil bacterial communities, with soil nitrate nitrogen content being the main environmental factor driving the change in soil bacterial community diversity. Comprehensive analysis revealed that water and nitrogen management influenced soil bacterial community structure and enzyme activity by changing the physical and chemical properties of the soil. The key parameters affecting soil enzyme activity were bacterial community diversity and soil pH, and it could be inhibited by a decrease in soil pH. The increased diversity of the bacterial community was beneficial to the enhancement of soil enzyme activity. |
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