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| Residual characteristics of organophosphorus pesticides in sediments of rice-crayfish water and mechanisms driving phosphorus cycling |
| Received:June 17, 2022 |
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| KeyWord:rice-crayfish water;organophosphorus pesticide;phosphorus cycle;microorganism;functional gene |
| Author Name | Affiliation | E-mail | | QU Mengjie | School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, China
College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China | | | HAN Yucheng | School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, China | | | WAN Zhipeng | School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, China | | | TAN Yan | School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, China | | | AN Jiaqi | College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China | | | MEI Yunjun | School of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, China | | | LIU Guanglong | College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China | liugl@mail.hzau.edu.cn |
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| Abstract: |
| To investigate the mechanisms through which organophosphorus pesticides drive the phosphorus cycle in rice-crayfish water, we used a combination of field sampling and indoor analysis to determine the organophosphorus pesticide contents, microbial community structure, and phosphorus cycle functional gene abundance in sediments, and we analyzed the relationship between organophosphorus pesticides and functional phosphorus-cycle genes using redundancy analysis(RDA) and Spearman correlation test,and that the highest detection rates were 97.22% for dimethoate, 22.22% for dichlorvos, 2.78% for parathion-methyl; we did not detect malathion or chlorpyrifos was not detected in the sediment. During the dry and wet periods, the sediment concentrations reached 1.42 mg·kg-1 and 0.46 mg·kg-1 for dimethoate and 1.37 mg·kg-1 and 0.05 mg·kg-1 for dichlorvos, respectively. In all sediments, the most important phosphorus cycling processes were as follows:the ppx gene was involved in the hydrolysis of polyphosphate by Proteobacteria and Omnitrophicaeota, the phoD gene was involved in organophosphorus mineralization, and the phnK gene was involved in phosphate use by Gemmatimonadetes. Organophosphorus pesticides did not affect the most important phosphorus cycling processes, but an increase in the concentration of dimethoate significantly increased the expression of cphy(cysteine phosphatase phytase) genes(P<0.01). An increase in the concentration of dichlorvos significantly decreased the expressions of pqqC(pyrroloquinoline-quinone synthase) and bpp(β-propeller phytase-propeller phytase) genes(P<0.05). Therefore, in the sediments of rice-crayfish water, organophosphorus pesticides dimethoate and dichlorvos affected the phosphorus cycling process mainly by altering inorganic phosphorus solubilization and phytate mineralization. |
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