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| Estrogen removal and its mechanism during anaerobic digestion of pig manure |
| Received:March 21, 2022 |
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| KeyWord:anaerobic digestion;estrogen;dissolved organic matter;microbial community structure |
| Author Name | Affiliation | E-mail | | ZHUANG Jie | School of Environmental & Safety Engineering, Changzhou University, Changzhou 213164, China | | | ZHAO Bincheng | School of Environmental & Safety Engineering, Changzhou University, Changzhou 213164, China | | | XUE Jinhui | School of Environmental & Safety Engineering, Changzhou University, Changzhou 213164, China | | | ZHANG Wenyi | School of Environmental & Safety Engineering, Changzhou University, Changzhou 213164, China | zwy@cczu.edu.cn |
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| Abstract: |
| To investigate the estrogen removal efficiency and its mechanism during the anaerobic digestion of pig manure, four estrogens (estrone E1, estradiol E2, estriol E3, and ethinylestradiol EE2) in anaerobic digestion liquid were tested periodically. Fourier transform infrared spectroscopy, three-dimensional fluorescence spectroscopy combined with fluorescence region integration, and high-throughput sequencing of the 16S rRNA gene were used to analyze the characteristic vibration peaks of infrared functional groups, dissolved organic matter(DOM), and microbial community structure in the manure. The results showed that under a temperature of 35℃ for 30 days and 6% solid concentration condition, the degradation rates of the four estrogens by anaerobic digestion were E2>E3>E1>EE2, and the removal efficiency rates were 28.62%, 25.83%, 19.14% and 11.81%, respectively. The degradation laws were consistent with the first-order kinetic degradation model for organic matter. After the addition of estrogen, changes occurred in the microbial community of the manure; however, normal anaerobic digestion was maintained. Anaerobic digestion could also be combined with increasing estrogen humic acid soluble organic matter content and amide, hydroxyl, and carboxyl adsorption sites, and highly efficient estrogen degradation bacteria. This study speculated that the removal mechanism of estrogen from biogas slurry included three aspects:adsorption of pig manure particles, adsorption of dissolved organic matter, and efficient metabolism of estrogen-degrading bacteria. |
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