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| Ionomic studies of rice seedling differential responses to arsenite stresses |
| Received:August 18, 2022 |
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| KeyWord:arsenic|Oryza sativa|ionomic|cluster analysis|principal component analysis |
| Author Name | Affiliation | E-mail | | ZENG Jin | College of Forestry, Henan Agricultural University, Zhengzhou 450002, China | | | ZANG Min | College of Forestry, Henan Agricultural University, Zhengzhou 450002, China | | | ZHANG Ruiqi | College of Forestry, Henan Agricultural University, Zhengzhou 450002, China | | | LI Limei | College of Forestry, Henan Agricultural University, Zhengzhou 450002, China | | | MA Li | College of Forestry, Henan Agricultural University, Zhengzhou 450002, China | malizhk@henau.edu.cn |
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| Abstract: |
| Ionomic profiles of plants display correlations with the stress inducing heavy metals to which they are exposed. Investigation of ionomic variation can provide insights into the mechanisms that promote element uptake or detoxification in plants. In this research, nine main rice varieties obtained from Linwu County, Hunan Province were treated with arsenate during hydroponic growth. The elements P, K, Mg, Ca, Mn, Fe, Cu, Zn, As, Cr, Co, Cd, Ba, Pb, and Sr in shoots and roots of rice seedlings subjected to As(Ⅲ) stress were quantitatively analyzed by ICP-MS. Associations between the ionome and arsenic in each tissue were further clarified using multivariate statistical analysis. Arsenic increased in both roots and shoots with increasing concentration of As(Ⅲ) exposure, and roots were more likely to accumulate arsenic than shoots. Fe, Ba, Pb, and Sr showed the same distribution trend as As, whereas the nutrient elements（P, K, Mg, Ca, and Mn）accumulated to higher levels in shoots than roots. The effects on distribution of each element in the two tissues of rice seedlings varied with stress condition, indicating that external As stress interfered with ion distribution in rice tissues. Cluster heat map and principal component analyses showed that ion groups appeared separately in shoots and roots, with Mg, K, P, Mn, Ca, Cr, Co, and Zn tending to be enriched in shoots, while Fe, Sr, Pb, Ba, Cu, As, and Cd tended to be enriched in roots. Multi-set significant correlations among elements could be found in rice tissues. As correlated positively with Fe, Cu, Cd, Ba, Pb, and Sr in roots and shoots（P<0.05）, while a significant（P<0.05）negative correlation could be found between As and P, K, Mg, Ca, and Mn, suggesting that ion groupings in rice exert regulatory effects on As migration and accumulation. These study results are expected to provide theoretical references for reducing arsenic contamination in rice. |
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