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| Screening of microplastic degrading bacteria and evaluation of their performance |
| Received:June 23, 2022 |
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| KeyWord:microplastics|environmental pollution|polyethylene|biodegradable|degradation performance |
| Author Name | Affiliation | E-mail | | ZHU Huihui | College of Life Science and Agriculture Engineering, Nanyang Normal University, Nanyang 473061, China
Institute of Plant Nutrition, Resources and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China | | | ZHANG Shubin | Institute of Plant Nutrition, Resources and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China | | | ZOU Guoyuan | Institute of Plant Nutrition, Resources and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China | | | XIAO Qiang | Institute of Plant Nutrition, Resources and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China | | | LIU Dongsheng | Institute of Plant Nutrition, Resources and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China | | | LIANG Zi'an | College of Life Science and Agriculture Engineering, Nanyang Normal University, Nanyang 473061, China | liangzian1965@163.com | | LIU Jianbin | Institute of Plant Nutrition, Resources and Environment, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China | liujianbin@baafs.net.cn |
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| Abstract: |
| To reduce the impact of microplastics on the environment, a five-point sampling method was used to collect soil from a longterm coated plot of Beijing Academy of Agriculture and Forestry Sciences in September 2020. A group of microplastic degrading bacteria, named ZH -5, was obtained using the enrichment culture method. The composition diversity and growth characteristics of ZH-5 were analyzed by high-throughput sequencing technology and Biolog-Eco microplate method. The degradation potential of ZH-5 for microplastics was evaluated by the weight loss method, scanning electron microscopy, and fourier infrared spectroscopy. The toxic effects of ZH-5 on crop seeds were investigated using the seed germination test. The results showed that the microplastic biodegradation flora consisted of nine phyla, belonging to 94 genera. Proteobacteria and Actinomycetes, which were the dominant phylum, accounted for 60.34% and 16.86%, respectively. The main bacteria were Noviherbaspirillum sp.（29.81%）, Ammoniphilus sp.（16.28%）, and Pseudomonas sp.（11.76%）. After 60 days of culture, the degradation rate of ZH-5 for polyethylene reached 2.86%, which showed a significant growth-promoting effect on crop seeds. Compared with the P0 treatment（treatment without bacterial solution）, the P1 treatment （inoculated 100 times diluted bacterial solution）promoted the growth rate of cucumber, radish, melon, and cantaloupe root length by 31.38%, 108.13%, 57.59% and 223.03%, respectively. The results show that ZH-5 has the potential to degrade polyethylene microplastics, and has certain research significance in reducing environmental pollution caused by microplastics. |
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