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| Effects of human urine application on lettuce quality traits and soil bacterial community structure |
| Received:August 10, 2022 |
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| KeyWord:human urine|bacteria community structure|lettuce|quality trait |
| Author Name | Affiliation | E-mail | | YU Guangquan | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | WEI Xiaocheng | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | 13752221539@163.com | | WANG Qian | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | WANG Qiang | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | ZHANG Chunxue | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | YANG Bo | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | ZHENG Xiangqun | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | zhengxiangqun@126.com |
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| Abstract: |
| Human urine, as a traditional organic liquid fertilizer, has wide application prospects. To investigate the effects of human urine application on soil microbial community structure, we conducted a potted lettuce（Lactuca sativa L.）experiment in a greenhouse with four different fertilizer applications：0 mL·kg-1（CK）, 13 mL·kg-1（T1）, 26 mL·kg-1（T2）and 52 mL·kg-1（T3）human urine. We measured the yield and quality traits of lettuce in these treatments. The results showed that compared with CK, the fresh weight of T1 and T2 lettuce increased 56.75% and 100.01%, respectively; and the soluble sugar content increased by 90.63% and 210.94%, respectively; but the Vc content decreased by 13.93% and 26.62%, respectively. Moreover, in T3, the lettuce did not grow. Compared with CK, the application of urine（T1-T3）increased soil TN, TP, AP, AK, NH+4-N, and SOM contents by 2.22%-37.78%, 7.94%-20.63%, 22.42%-128.90%, 7.71%-83.70%, 84.62%-159.62%, and 3.64%-7.52%, respectively, but also increased soil EC by 13.56%-161.02%; soil C/N and pH decreased by 2.77%-21.90% and 2.10%-10.28%. With the increase in human urine dosage, the total number of OTUs decreased, the Shannon index decreased, and the Simpson index increased. With the increase in human urine dosage, the relative abundance of Proteobacteria and Gemmatimonadetes in the soil gradually increased, while the relative abundance of Firmicutes gradually decreased, and the relative abundance of Actinobacteria, Chloroflexi and Acidobacteria in each treatment showed a trend of increasing and then decreasing. We obtained the highest values from T1 lettuce, where they were increased by 14.71%, 2.06%, and 16.17%, respectively, compared with those of CK. In summary, within a certain range, the application of human urine can substantially increase the fresh weight and soluble sugar content of lettuce, and improve the soil nutrient content, but excessive application affects the structure of the soil bacterial community and reduces the diversity and abundance of the bacterial community, and thus lettuce does not grow normally. |
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