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| Pollution characteristics of antibiotic resistance genes in ecological recirculating aquaculture ponds |
| Received:May 16, 2022 |
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| KeyWord:ecological recirculating aquaculture system|pond aquaculture|antibiotic resistance genes（ARGs）|microbal community|pollution characteristics |
| Author Name | Affiliation | E-mail | | QU Jiangqi | Beijing Key Laboratory of Fishery Biotechnology, Fisheries Science Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100068, China | | | ZHANG Qingjing | Beijing Key Laboratory of Fishery Biotechnology, Fisheries Science Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100068, China | zhangqjhbs@126.com | | WU Yanfei | Beijing Key Laboratory of Fishery Biotechnology, Fisheries Science Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100068, China | | | YU Wenyu | Beijing Key Laboratory of Fishery Biotechnology, Fisheries Science Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100068, China
Tianjin Agricultural College, Tianjin 300392, China | | | ZHAO Meng | Beijing Key Laboratory of Fishery Biotechnology, Fisheries Science Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100068, China | | | ZHU Hua | Beijing Key Laboratory of Fishery Biotechnology, Fisheries Science Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100068, China | |
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| Abstract: |
| To explore the characteristics of Antibiotic resistance genes（ARGs）pollution in the ecological recirculating aquaculture ponds, this study used metagenomic technology to detect and analyze the ARG profiles and their relationship with microbial communities and environmental variables. The results showed that a total of 21 types and 1092 subtypes of ARGs were detected in aquaculture ponds, and the pond sediment was the main reservoir of ARGs. The resistance genes macB, tetA（58）, and nov showed higher relative abundances in the pond. Multidrug and antibiotic efflux pumps were the most important types of ARGs and resistance mechanisms, respectively. There were significant differences in microbial community composition between the water column and bottom mud of the aquaculture pond（P<0.05）. There was a significant positive correlation between ARGs and Proteobacteria, Cyanobacteria, and Actinobacteria, indicating that ARGs were transferred horizontally among these different bacterial groups. At the same time, microorganisms also provided favorable conditions for the persistence and diffusion of ARGs in the aquaculture system. In addition, ammonia nitrogen, nitrite nitrogen, and nitrate nitrogen were major environmental factors that affected the distribution characteristics of ARGs and the composition of microbial communities in aquaculture ponds. The results of this study determined the possible background value of ARGs in the ecological recirculating aquaculture ponds. They also provided quantitative information that the tailwater had a high risk of ARGs transmission. The results provided data support for the further optimization and upgrading of ecological recirculating aquaculture systems, reducing ARGs pollution, and protecting the aquaculture environment. |
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